tetano
Editor, Senior Moderator
Vaccine
In Press, Uncorrected Proof - Note to users
doi:10.1016/j.vaccine.2011.09.110 | How to Cite or Link Using DOI
Permissions & Reprints
Antibody responses and cross protection against lethal influenza A viruses differ between the sexes in C57BL/6 mice
Maria E. Lorenzoa, c, 1, Andrea Hodgsona, 1, Dionne P. Robinsona, Jenifer B. Kaplana, 2, Andrew Pekosza, Sabra L. Kleina, b, Corresponding Author Contact Information, E-mail The Corresponding Author
Purchase
a The W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States
b Department of Biochemistry and Molecular Biology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States
c Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, MD, United States
Received 7 April 2011; revised 22 July 2011; Accepted 23 September 2011. Available online 6 October 2011.
Abstract
A mouse model was used to determine if protective immunity to influenza A virus infection differs between the sexes. The median lethal dose of H1N1 or H3N2 was lower for na?ve females than males. After a sublethal, primary infection with H1N1 or H3N2, females and males showed a similar transient morbidity, but females generated more neutralizing and total anti-influenza A virus antibodies. Immunized males and females showed similar protection against secondary challenge with a homologous virus, but males experienced greater morbidity and had higher lung viral titers after infection with a lethal dose of heterologous virus. Females develop stronger humoral immune responses and greater cross protection against heterosubtypic virus challenge.
Highlights
► We developed a small animal model to determine if protective immunity against influenza A viruses is higher in females than males. ► We examined humoral immune responses to primary sublethal infection with H1N1 or H3N2 viruses and virus titers and morbidity following secondary lethal challenge with homologous or heterologous virus. ► Females mount higher neutralizing and total anti-influenza A virus antibodies than males. ► Males and females are equally protected against homologous virus challenge, but males experience greater morbidity and have higher viral titers in their lungs after infection with a lethal dose of heterologous virus. ► We conclude that females develop greater cross protection against influenza A viruses than males.
http://www.sciencedirect.com/science/article/pii/S0264410X11015520
In Press, Uncorrected Proof - Note to users
doi:10.1016/j.vaccine.2011.09.110 | How to Cite or Link Using DOI
Permissions & Reprints
Antibody responses and cross protection against lethal influenza A viruses differ between the sexes in C57BL/6 mice
Maria E. Lorenzoa, c, 1, Andrea Hodgsona, 1, Dionne P. Robinsona, Jenifer B. Kaplana, 2, Andrew Pekosza, Sabra L. Kleina, b, Corresponding Author Contact Information, E-mail The Corresponding Author
Purchase
a The W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States
b Department of Biochemistry and Molecular Biology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States
c Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, MD, United States
Received 7 April 2011; revised 22 July 2011; Accepted 23 September 2011. Available online 6 October 2011.
Abstract
A mouse model was used to determine if protective immunity to influenza A virus infection differs between the sexes. The median lethal dose of H1N1 or H3N2 was lower for na?ve females than males. After a sublethal, primary infection with H1N1 or H3N2, females and males showed a similar transient morbidity, but females generated more neutralizing and total anti-influenza A virus antibodies. Immunized males and females showed similar protection against secondary challenge with a homologous virus, but males experienced greater morbidity and had higher lung viral titers after infection with a lethal dose of heterologous virus. Females develop stronger humoral immune responses and greater cross protection against heterosubtypic virus challenge.
Highlights
► We developed a small animal model to determine if protective immunity against influenza A viruses is higher in females than males. ► We examined humoral immune responses to primary sublethal infection with H1N1 or H3N2 viruses and virus titers and morbidity following secondary lethal challenge with homologous or heterologous virus. ► Females mount higher neutralizing and total anti-influenza A virus antibodies than males. ► Males and females are equally protected against homologous virus challenge, but males experience greater morbidity and have higher viral titers in their lungs after infection with a lethal dose of heterologous virus. ► We conclude that females develop greater cross protection against influenza A viruses than males.
http://www.sciencedirect.com/science/article/pii/S0264410X11015520