tetano
Editor, Senior Moderator
Antimicrob Agents Chemother. 2017 Apr 17. pii: AAC.00539-17. doi: 10.1128/AAC.00539-17. [Epub ahead of print]
[h=1]Anti-influenza activity of Bacillus subtilis probiotic strain.[/h] Starosila D[SUP]1[/SUP], Rybalko S[SUP]1[/SUP], Varbanetz L[SUP]2[/SUP], Ivanskaya N[SUP]1[/SUP], Sorokulova I[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Among Bacillus bacteria, B. subtilis is the most productive species of antimicrobial compounds. In this study, we analyzed the activity of probiotic strain B. subtilis 3 against influenza virus. The antiviral effect of this strain has been demonstrated in vitro and in vivo New peptide P18 produced by probiotic strain was isolated, purified, chemically synthesized, and characterized. Cytotoxicity studies demonstrated no toxic effect of P18 on Madin-Darby canine kidney (MDCK) cells, even in the highest tested concentration (100 μg/mL). Complete inhibition of influenza virus in vitro was observed at concentrations 12.5 - 100 μg/mL. Protective effect of P18 in mice was comparable with Tamiflu. Further study will assess the potential of peptide P18 as antiviral compound and as a promising candidate for the development of new antiviral vaccines.
Copyright ? 2017 American Society for Microbiology.
PMID: 28416546 DOI: 10.1128/AAC.00539-17
[h=1]Anti-influenza activity of Bacillus subtilis probiotic strain.[/h] Starosila D[SUP]1[/SUP], Rybalko S[SUP]1[/SUP], Varbanetz L[SUP]2[/SUP], Ivanskaya N[SUP]1[/SUP], Sorokulova I[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Among Bacillus bacteria, B. subtilis is the most productive species of antimicrobial compounds. In this study, we analyzed the activity of probiotic strain B. subtilis 3 against influenza virus. The antiviral effect of this strain has been demonstrated in vitro and in vivo New peptide P18 produced by probiotic strain was isolated, purified, chemically synthesized, and characterized. Cytotoxicity studies demonstrated no toxic effect of P18 on Madin-Darby canine kidney (MDCK) cells, even in the highest tested concentration (100 μg/mL). Complete inhibition of influenza virus in vitro was observed at concentrations 12.5 - 100 μg/mL. Protective effect of P18 in mice was comparable with Tamiflu. Further study will assess the potential of peptide P18 as antiviral compound and as a promising candidate for the development of new antiviral vaccines.
Copyright ? 2017 American Society for Microbiology.
PMID: 28416546 DOI: 10.1128/AAC.00539-17