tetano
Editor, Senior Moderator
BMC Microbiol. 2017 Sep 11;17(1):191. doi: 10.1186/s12866-017-1097-0.
[h=1]Annexin A2 (ANXA2) interacts with nonstructural protein 1 and promotes the replication of highly pathogenic H5N1 avian influenza virus.[/h] Ma Y[SUP]1[/SUP], Sun J[SUP]1[/SUP], Gu L[SUP]1[/SUP], Bao H[SUP]1[/SUP], Zhao Y[SUP]1[/SUP], Shi L[SUP]2[/SUP], Yao W[SUP]2[/SUP], Tian G[SUP]1[/SUP], Wang X[SUP]3[/SUP], Chen H[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antibody against NS1 protein; In the current research study, using this antibody, we immunoprecipitated host proteins that interact with NS1 to better understand the roles played by NS1 in communications between virus and host.
[h=4]RESULTS:[/h] Co-immunoprecipitation experiments identified annexin A2 (ANXA2) as a target molecule interacting with NS1. Results from confocal laser scanning microscopy indicated that NS1 co-localized with ANXA2 in the cell cytoplasm. Overexpression of ANXA2 significantly increased the titer of H5N1 subtype HPAIV, whereas siRNA-mediated knockdown of ANXA2 markedly inhibited the expression of viral proteins and reduced the progeny virus titer.
[h=4]CONCLUSIONS:[/h] Our results indicate that ANXA2 interacts with NS1 and ANXA2 expression increases HPAIV replication.
[h=4]KEYWORDS:[/h] ANXA2; H5N1 subtype HPAIV; NS1; Viral replication
PMID: 28893180 DOI: 10.1186/s12866-017-1097-0
[h=1]Annexin A2 (ANXA2) interacts with nonstructural protein 1 and promotes the replication of highly pathogenic H5N1 avian influenza virus.[/h] Ma Y[SUP]1[/SUP], Sun J[SUP]1[/SUP], Gu L[SUP]1[/SUP], Bao H[SUP]1[/SUP], Zhao Y[SUP]1[/SUP], Shi L[SUP]2[/SUP], Yao W[SUP]2[/SUP], Tian G[SUP]1[/SUP], Wang X[SUP]3[/SUP], Chen H[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antibody against NS1 protein; In the current research study, using this antibody, we immunoprecipitated host proteins that interact with NS1 to better understand the roles played by NS1 in communications between virus and host.
[h=4]RESULTS:[/h] Co-immunoprecipitation experiments identified annexin A2 (ANXA2) as a target molecule interacting with NS1. Results from confocal laser scanning microscopy indicated that NS1 co-localized with ANXA2 in the cell cytoplasm. Overexpression of ANXA2 significantly increased the titer of H5N1 subtype HPAIV, whereas siRNA-mediated knockdown of ANXA2 markedly inhibited the expression of viral proteins and reduced the progeny virus titer.
[h=4]CONCLUSIONS:[/h] Our results indicate that ANXA2 interacts with NS1 and ANXA2 expression increases HPAIV replication.
[h=4]KEYWORDS:[/h] ANXA2; H5N1 subtype HPAIV; NS1; Viral replication
PMID: 28893180 DOI: 10.1186/s12866-017-1097-0