tetano
Editor, Senior Moderator
Ann Transl Med
. 2020 Dec;8(24):1631.
doi: 10.21037/atm-20-4060.
Chinese Expert Consensus on the Nucleic Acid Detection of SARS-CoV-2
Zijie Liu[SUP] 1 2 [/SUP], Yongqing Tong[SUP] 3 [/SUP], Jun Wu[SUP] 4 [/SUP], Lutao Du[SUP] 5 [/SUP], Chaojun Wei[SUP] 6 [/SUP], Wei Cui[SUP] 7 [/SUP], Yongtong Cao[SUP] 8 [/SUP], Ming Chen[SUP] 9 [/SUP], Zhen Cai[SUP] 10 [/SUP], Wei Chen[SUP] 11 [/SUP], Haitao Ding[SUP] 12 [/SUP], Ming Guan[SUP] 13 [/SUP], Wei Guo[SUP] 14 [/SUP], Chunfang Gao[SUP] 15 [/SUP], Xiaoke Hao[SUP] 16 [/SUP], Chengjin Hu[SUP] 17 [/SUP], Shan Huang[SUP] 18 [/SUP], Yanfang Jiang[SUP] 19 [/SUP], Jinming Li[SUP] 20 [/SUP], Ping Li[SUP] 21 [/SUP], Zhuo Li[SUP] 22 [/SUP], Liang Ming[SUP] 23 [/SUP], Shiyang Pan[SUP] 24 [/SUP], Zuojun Shen[SUP] 25 [/SUP], Jianrong Su[SUP] 26 [/SUP], Ziyong Sun[SUP] 27 [/SUP], Hui Wang[SUP] 28 [/SUP], Junjun Wang[SUP] 29 [/SUP], Bin Xu[SUP] 30 [/SUP], Nong Yu[SUP] 31 [/SUP], Lei Zheng[SUP] 10 [/SUP], Yi Zhang[SUP] 32 [/SUP], Xin Zhang[SUP] 33 [/SUP], Ying Zhang[SUP] 34 [/SUP], Yong Duan[SUP] 1 2 [/SUP], Chengbin Wang[SUP] 34 [/SUP]
Affiliations
Abstract
The coronavirus disease 2019 (COVID-19) has already become a pandemic wherein the infection's timely diagnosis has proven beneficial to patient treatment and disease control. Nucleic acid detection has been the primary laboratory diagnostic method for the detection of SARS-CoV-2. To ensure laboratory staff safety and quality nucleic acid testing, the Chinese Society of Laboratory Medicine formulated this consensus, based on the Chinese National Recommendations and previous literature for nucleic acid detection. A working group comprises 34 hospital professionals experience with real-time polymerase chain reactions (PCR) testing for SARS-CoV-2 drafted guidance statements during online discussions. A modified Delphi methodology was used in forming a consensus among a wider group of hospital professionals with SARS-CoV-2 detection experience. Guidance statements were developed for four categories: (I) specimen type, priority, collecting, transportation and receiving; (II) nucleic acid isolation and amplification; (III) quality control; (IV) biosafety management and decontamination. The modified Delphi voting process included a total of 29 guidance statements and final agreement. Consensus was reached after two rounds of voting. Recommendations were established for the detection of SARS-CoV-2 using real time PCR testing based on evidence and group consensus. The manuscript was evaluated against The Appraisal of Guidelines for Research & Evaluation Instrument (AGREE II) and was developed to aid medical laboratory staff in the detection of the ribonucleic acid (RNA) of SARS-CoV-2.
Keywords: SARS-CoV-2; coronavirus disease 2019 (COVID-19); nucleic acid; polymerase chain reaction (PCR).
. 2020 Dec;8(24):1631.
doi: 10.21037/atm-20-4060.
Chinese Expert Consensus on the Nucleic Acid Detection of SARS-CoV-2
Zijie Liu[SUP] 1 2 [/SUP], Yongqing Tong[SUP] 3 [/SUP], Jun Wu[SUP] 4 [/SUP], Lutao Du[SUP] 5 [/SUP], Chaojun Wei[SUP] 6 [/SUP], Wei Cui[SUP] 7 [/SUP], Yongtong Cao[SUP] 8 [/SUP], Ming Chen[SUP] 9 [/SUP], Zhen Cai[SUP] 10 [/SUP], Wei Chen[SUP] 11 [/SUP], Haitao Ding[SUP] 12 [/SUP], Ming Guan[SUP] 13 [/SUP], Wei Guo[SUP] 14 [/SUP], Chunfang Gao[SUP] 15 [/SUP], Xiaoke Hao[SUP] 16 [/SUP], Chengjin Hu[SUP] 17 [/SUP], Shan Huang[SUP] 18 [/SUP], Yanfang Jiang[SUP] 19 [/SUP], Jinming Li[SUP] 20 [/SUP], Ping Li[SUP] 21 [/SUP], Zhuo Li[SUP] 22 [/SUP], Liang Ming[SUP] 23 [/SUP], Shiyang Pan[SUP] 24 [/SUP], Zuojun Shen[SUP] 25 [/SUP], Jianrong Su[SUP] 26 [/SUP], Ziyong Sun[SUP] 27 [/SUP], Hui Wang[SUP] 28 [/SUP], Junjun Wang[SUP] 29 [/SUP], Bin Xu[SUP] 30 [/SUP], Nong Yu[SUP] 31 [/SUP], Lei Zheng[SUP] 10 [/SUP], Yi Zhang[SUP] 32 [/SUP], Xin Zhang[SUP] 33 [/SUP], Ying Zhang[SUP] 34 [/SUP], Yong Duan[SUP] 1 2 [/SUP], Chengbin Wang[SUP] 34 [/SUP]
Affiliations
- PMID: 33490143
- PMCID: PMC7812184
- DOI: 10.21037/atm-20-4060
Abstract
The coronavirus disease 2019 (COVID-19) has already become a pandemic wherein the infection's timely diagnosis has proven beneficial to patient treatment and disease control. Nucleic acid detection has been the primary laboratory diagnostic method for the detection of SARS-CoV-2. To ensure laboratory staff safety and quality nucleic acid testing, the Chinese Society of Laboratory Medicine formulated this consensus, based on the Chinese National Recommendations and previous literature for nucleic acid detection. A working group comprises 34 hospital professionals experience with real-time polymerase chain reactions (PCR) testing for SARS-CoV-2 drafted guidance statements during online discussions. A modified Delphi methodology was used in forming a consensus among a wider group of hospital professionals with SARS-CoV-2 detection experience. Guidance statements were developed for four categories: (I) specimen type, priority, collecting, transportation and receiving; (II) nucleic acid isolation and amplification; (III) quality control; (IV) biosafety management and decontamination. The modified Delphi voting process included a total of 29 guidance statements and final agreement. Consensus was reached after two rounds of voting. Recommendations were established for the detection of SARS-CoV-2 using real time PCR testing based on evidence and group consensus. The manuscript was evaluated against The Appraisal of Guidelines for Research & Evaluation Instrument (AGREE II) and was developed to aid medical laboratory staff in the detection of the ribonucleic acid (RNA) of SARS-CoV-2.
Keywords: SARS-CoV-2; coronavirus disease 2019 (COVID-19); nucleic acid; polymerase chain reaction (PCR).