tetano
Editor, Senior Moderator
Ann Clin Biochem
. 2024 Oct 22:45632241297819.
doi: 10.1177/00045632241297819. Online ahead of print. Application of surface plasmon resonance imaging in the high-throughput detection of influenza virus
Haixiang Zhang[SUP] 1 [/SUP], Jingying Sun[SUP] 2 [/SUP], Chunyan Guo[SUP] 2 [/SUP], Qing Feng[SUP] 2 [/SUP], Yan Li[SUP] 2 [/SUP], Xiangrong Zhao[SUP] 2 [/SUP], Lijun Sun[SUP] 2 [/SUP], Cuixiang Xu[SUP] 2 [/SUP]
Affiliations
Objective: To evaluate the application effect of SPRi monoclonal antibody (mAb) chip in the detection of influenza virus antigen in complex mixtures.
Methods: A total of 115 strains of mAbs against different subtypes (H1N1, H5N1, A1, A3, B, H7N9, H9N2, and H3N2) of influenza virus were prepared. The chip of mAbs against influenza virus was prepared by surface plasmonic resonance imaging (SPRi) technology, which was used for the detection of influenza virus supernatant, and compared with the traditional antigen capture ELISA method.
Results: Comparative studies have shown that traditional antigen capture ELISA methods have a higher sensitivity (86.8% (46/53) vs. 46.5% (46/99); z = 4.84, P < 0.001), while the SPRi chip methods present a significantly higher specificity (56.3% (9/16) vs. 14.5% (9/62); z = 3.54, P < 0.001). The SPRi chip detection method for influenza virus antibodies can well reflect the specific binding characteristics of influenza virus antigens and antibodies.
Conclusion: The SPRi mAb chip can be used for the detection of specific pathogenic microorganisms or viral proteins in complex mixtures such as influenza virus supernatant. It has significant advantages of label free, real-time, high-throughput, and good specificity, and can play an important role in disease diagnosis and infectious disease prevention and control.
Keywords: Analytes; Immunoassay; Laboratory methods; Monoclonal antibodies; Proteins.
. 2024 Oct 22:45632241297819.
doi: 10.1177/00045632241297819. Online ahead of print. Application of surface plasmon resonance imaging in the high-throughput detection of influenza virus
Haixiang Zhang[SUP] 1 [/SUP], Jingying Sun[SUP] 2 [/SUP], Chunyan Guo[SUP] 2 [/SUP], Qing Feng[SUP] 2 [/SUP], Yan Li[SUP] 2 [/SUP], Xiangrong Zhao[SUP] 2 [/SUP], Lijun Sun[SUP] 2 [/SUP], Cuixiang Xu[SUP] 2 [/SUP]
Affiliations
- PMID: 39439152
- DOI: 10.1177/00045632241297819
Objective: To evaluate the application effect of SPRi monoclonal antibody (mAb) chip in the detection of influenza virus antigen in complex mixtures.
Methods: A total of 115 strains of mAbs against different subtypes (H1N1, H5N1, A1, A3, B, H7N9, H9N2, and H3N2) of influenza virus were prepared. The chip of mAbs against influenza virus was prepared by surface plasmonic resonance imaging (SPRi) technology, which was used for the detection of influenza virus supernatant, and compared with the traditional antigen capture ELISA method.
Results: Comparative studies have shown that traditional antigen capture ELISA methods have a higher sensitivity (86.8% (46/53) vs. 46.5% (46/99); z = 4.84, P < 0.001), while the SPRi chip methods present a significantly higher specificity (56.3% (9/16) vs. 14.5% (9/62); z = 3.54, P < 0.001). The SPRi chip detection method for influenza virus antibodies can well reflect the specific binding characteristics of influenza virus antigens and antibodies.
Conclusion: The SPRi mAb chip can be used for the detection of specific pathogenic microorganisms or viral proteins in complex mixtures such as influenza virus supernatant. It has significant advantages of label free, real-time, high-throughput, and good specificity, and can play an important role in disease diagnosis and infectious disease prevention and control.
Keywords: Analytes; Immunoassay; Laboratory methods; Monoclonal antibodies; Proteins.