tetano
Editor, Senior Moderator
Animal Model Exp Med
. 2023 Jul 10.
doi: 10.1002/ame2.12336. Online ahead of print. The protection of CoronaVac against the infection of wild-type SARS-CoV-2 (WH-09) or Omicron variant in nude-hACE2 mice
Kaili Lin[SUP] 1 [/SUP], Meixuan Liu[SUP] 1 [/SUP], Lu Sun[SUP] 1 [/SUP], Hanjun Fu[SUP] 1 [/SUP], Hongwei Qiao[SUP] 1 [/SUP], Shunyi Wang[SUP] 1 [/SUP], Sidan Pan[SUP] 1 [/SUP], Hong Gao[SUP] 1 [/SUP]
Affiliations
Background: Immunocompromised individuals have an increased risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and severe outcomes, but we pay less attention to these people. Athymic nude mice are a murine strain with a spontaneous deficiency of the Foxn1 gene, which can result in thymic degeneration or its absence, leading to immunosuppression and a decrease in the number of T cells, and are widely used in preclinical evaluations of disease in immunocompromised populations.
Methods: We investigated the protection of the coronavirus disease 2019 (COVID-19) inactivated vaccine (CoronaVac) against the infection of wild-type SARS-CoV-2 (WH-09) or Omicron variant utilizing a hybrid-type nude-hACE2 mouse model.
Results: Compared with nude-hACE2/W mice, the viral load in the brain and lung tissue of nude-hACE2 mice (nude-hACE2/WV) infected with WH-09 after vaccination significantly decreased, and the histopathological changes were also reduced. The viral load in the brain and lung tissue of nude-hACE2 mice (nude-hACE2/OV) infected with the Omicron variant after vaccination was lower than that in nude-hACE2/O, but histopathological symptoms did not improve significantly.
Conclusion: CoronaVac provides some protection against infection of both WH-09 and the Omicron variant in the nude-hACE2 mice. Our findings aimed to provide a reference for vaccination against SARS-CoV-2 in immunocompromised populations.
Keywords: immunocompromised; nude-hACE2 mice; protection; severe acute respiratory syndrome coronavirus 2.
. 2023 Jul 10.
doi: 10.1002/ame2.12336. Online ahead of print. The protection of CoronaVac against the infection of wild-type SARS-CoV-2 (WH-09) or Omicron variant in nude-hACE2 mice
Kaili Lin[SUP] 1 [/SUP], Meixuan Liu[SUP] 1 [/SUP], Lu Sun[SUP] 1 [/SUP], Hanjun Fu[SUP] 1 [/SUP], Hongwei Qiao[SUP] 1 [/SUP], Shunyi Wang[SUP] 1 [/SUP], Sidan Pan[SUP] 1 [/SUP], Hong Gao[SUP] 1 [/SUP]
Affiliations
- PMID: 37431213
- DOI: 10.1002/ame2.12336
Background: Immunocompromised individuals have an increased risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and severe outcomes, but we pay less attention to these people. Athymic nude mice are a murine strain with a spontaneous deficiency of the Foxn1 gene, which can result in thymic degeneration or its absence, leading to immunosuppression and a decrease in the number of T cells, and are widely used in preclinical evaluations of disease in immunocompromised populations.
Methods: We investigated the protection of the coronavirus disease 2019 (COVID-19) inactivated vaccine (CoronaVac) against the infection of wild-type SARS-CoV-2 (WH-09) or Omicron variant utilizing a hybrid-type nude-hACE2 mouse model.
Results: Compared with nude-hACE2/W mice, the viral load in the brain and lung tissue of nude-hACE2 mice (nude-hACE2/WV) infected with WH-09 after vaccination significantly decreased, and the histopathological changes were also reduced. The viral load in the brain and lung tissue of nude-hACE2 mice (nude-hACE2/OV) infected with the Omicron variant after vaccination was lower than that in nude-hACE2/O, but histopathological symptoms did not improve significantly.
Conclusion: CoronaVac provides some protection against infection of both WH-09 and the Omicron variant in the nude-hACE2 mice. Our findings aimed to provide a reference for vaccination against SARS-CoV-2 in immunocompromised populations.
Keywords: immunocompromised; nude-hACE2 mice; protection; severe acute respiratory syndrome coronavirus 2.