tetano
Editor, Senior Moderator
Animal Model Exp Med
. 2024 May 27.
doi: 10.1002/ame2.12435. Online ahead of print. IL-37 possesses both anti-inflammatory and antiviral effects against Middle East respiratory syndrome coronavirus infection
Feifei Qi[SUP] 1 2 [/SUP], Yiwei Yan[SUP] 1 [/SUP], Qi Lv[SUP] 1 2 [/SUP], Mingya Liu[SUP] 1 [/SUP], Ming Liu[SUP] 1 [/SUP], Fengdi Li[SUP] 1 2 [/SUP], Ran Deng[SUP] 1 2 [/SUP], Xujian Liang[SUP] 1 2 [/SUP], Shuyue Li[SUP] 1 [/SUP], Guocui Mou[SUP] 1 [/SUP], Linlin Bao[SUP] 1 2 3 [/SUP]
Affiliations
Background: The aim was to elucidate the function of IL-37 in middle east respiratory syndrome coronavirus (MERS-CoV) infection, thereby providing a novel therapeutic strategy for managing the clinical treatment of inflammatory response caused by respiratory virus infection.
Methods: We investigated the development of MERS by infecting hDPP4 mice with hCoV-EMC (10[SUP]7[/SUP] TCID[SUB]50[/SUB] [50% tissue culture infectious dose]) intranasally. We infected A549 cells with MERS-CoV, which concurrently interfered with IL-37, detecting the viral titer, viral load, and cytokine expression at certain points postinfection. Meanwhile, we administered IL-37 (12.5 μg/kg) intravenously to hDPP4 mice 2 h after MERS-CoV-2 infection and collected the serum and lungs 5 days after infection to investigate the efficacy of IL-37 in MERS-CoV infection.
Results: The viral titer of MERS-CoV-infected A549 cells interfering with IL-37 was significantly reduced by 4.7-fold, and the viral load of MERS-CoV-infected hDPP4 mice was decreased by 59-fold in lung tissue. Furthermore, the administration of IL-37 suppressed inflammatory cytokine and chemokine (monocyte chemoattractant protein 1, interferon-γ, and IL-17A) expression and ameliorated the infiltration of inflammatory cells in hDPP4 mice.
Conclusion: IL-37 exhibits protective properties in severe pneumonia induced by MERS-CoV infection. This effect is achieved through attenuation of lung viral load, suppression of inflammatory cytokine secretion, reduction in inflammatory cell infiltration, and mitigation of pulmonary injury.
Keywords: MERS‐CoV; anti‐inflammation; interleukin‐37; pneumonia.
. 2024 May 27.
doi: 10.1002/ame2.12435. Online ahead of print. IL-37 possesses both anti-inflammatory and antiviral effects against Middle East respiratory syndrome coronavirus infection
Feifei Qi[SUP] 1 2 [/SUP], Yiwei Yan[SUP] 1 [/SUP], Qi Lv[SUP] 1 2 [/SUP], Mingya Liu[SUP] 1 [/SUP], Ming Liu[SUP] 1 [/SUP], Fengdi Li[SUP] 1 2 [/SUP], Ran Deng[SUP] 1 2 [/SUP], Xujian Liang[SUP] 1 2 [/SUP], Shuyue Li[SUP] 1 [/SUP], Guocui Mou[SUP] 1 [/SUP], Linlin Bao[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 38803038
- DOI: 10.1002/ame2.12435
Background: The aim was to elucidate the function of IL-37 in middle east respiratory syndrome coronavirus (MERS-CoV) infection, thereby providing a novel therapeutic strategy for managing the clinical treatment of inflammatory response caused by respiratory virus infection.
Methods: We investigated the development of MERS by infecting hDPP4 mice with hCoV-EMC (10[SUP]7[/SUP] TCID[SUB]50[/SUB] [50% tissue culture infectious dose]) intranasally. We infected A549 cells with MERS-CoV, which concurrently interfered with IL-37, detecting the viral titer, viral load, and cytokine expression at certain points postinfection. Meanwhile, we administered IL-37 (12.5 μg/kg) intravenously to hDPP4 mice 2 h after MERS-CoV-2 infection and collected the serum and lungs 5 days after infection to investigate the efficacy of IL-37 in MERS-CoV infection.
Results: The viral titer of MERS-CoV-infected A549 cells interfering with IL-37 was significantly reduced by 4.7-fold, and the viral load of MERS-CoV-infected hDPP4 mice was decreased by 59-fold in lung tissue. Furthermore, the administration of IL-37 suppressed inflammatory cytokine and chemokine (monocyte chemoattractant protein 1, interferon-γ, and IL-17A) expression and ameliorated the infiltration of inflammatory cells in hDPP4 mice.
Conclusion: IL-37 exhibits protective properties in severe pneumonia induced by MERS-CoV infection. This effect is achieved through attenuation of lung viral load, suppression of inflammatory cytokine secretion, reduction in inflammatory cell infiltration, and mitigation of pulmonary injury.
Keywords: MERS‐CoV; anti‐inflammation; interleukin‐37; pneumonia.