tetano
Editor, Senior Moderator
Enferm Infecc Microbiol Clin. 2015 Nov 24. pii: S0213-005X(15)00378-X. doi: 10.1016/j.eimc.2015.10.006. [Epub ahead of print]
[h=1]Analytical performance of the Alere? i Influenza A&B assay for the rapid detection of influenza viruses.[/h] Riazzo C[SUP]1[/SUP], P?rez-Ruiz M[SUP]2[/SUP], Sanbonmatsu-G?mez S[SUP]1[/SUP], Pedrosa-Corral I[SUP]1[/SUP], Guti?rrez-Fern?ndez J[SUP]1[/SUP], Navarro-Mar? JM[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The analytical performance of the new Alere? i Influenza A&B kit (AL-Flu) assay, based on isothermal nucleic acids amplification, was evaluated and compared with an antigen detection method, SD Bioline Influenza Virus Antigen Test (SDB), and an automated real-time RT-PCR, Simplexa? Flu A/B & VRS Direct assay (SPX), for detection of influenza viruses. An "in-house" RT-PCR was used as the reference method. Sensitivity of AL-Flu, SDB, and SPX was 71.7%, 34.8%, and 100%, respectively. Specificity was 100% for all techniques. The turnaround time was 13min for AL-Flu, 15min for SDB, and 75min for SPX. The Alere? i Influenza A&B assay is an optimal point-of-care assay for influenza diagnosis in clinical emergency settings, and is more sensitive and specific than antigen detection methods.
Copyright ? 2015 Elsevier Espa?a, S.L.U. y Sociedad Espa?ola de Enfermedades Infecciosas y Microbiolog?a Cl?nica. All rights reserved.
[h=4]KEYWORDS:[/h] Detecci?n r?pida; Gripe; Influenza; Molecular testing; M?todo molecular; Rapid detection
PMID: 26620605 [PubMed - as supplied by publisher]
[h=1]Analytical performance of the Alere? i Influenza A&B assay for the rapid detection of influenza viruses.[/h] Riazzo C[SUP]1[/SUP], P?rez-Ruiz M[SUP]2[/SUP], Sanbonmatsu-G?mez S[SUP]1[/SUP], Pedrosa-Corral I[SUP]1[/SUP], Guti?rrez-Fern?ndez J[SUP]1[/SUP], Navarro-Mar? JM[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The analytical performance of the new Alere? i Influenza A&B kit (AL-Flu) assay, based on isothermal nucleic acids amplification, was evaluated and compared with an antigen detection method, SD Bioline Influenza Virus Antigen Test (SDB), and an automated real-time RT-PCR, Simplexa? Flu A/B & VRS Direct assay (SPX), for detection of influenza viruses. An "in-house" RT-PCR was used as the reference method. Sensitivity of AL-Flu, SDB, and SPX was 71.7%, 34.8%, and 100%, respectively. Specificity was 100% for all techniques. The turnaround time was 13min for AL-Flu, 15min for SDB, and 75min for SPX. The Alere? i Influenza A&B assay is an optimal point-of-care assay for influenza diagnosis in clinical emergency settings, and is more sensitive and specific than antigen detection methods.
Copyright ? 2015 Elsevier Espa?a, S.L.U. y Sociedad Espa?ola de Enfermedades Infecciosas y Microbiolog?a Cl?nica. All rights reserved.
[h=4]KEYWORDS:[/h] Detecci?n r?pida; Gripe; Influenza; Molecular testing; M?todo molecular; Rapid detection
PMID: 26620605 [PubMed - as supplied by publisher]