tetano
Editor, Senior Moderator
Anal Chim Acta
. 2023 Feb 15;1242:340716.
doi: 10.1016/j.aca.2022.340716. Epub 2022 Dec 8.
Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70): Application in diagnosis/prognosis of coronavirus disease 2019 (COVID-19)
Masoud Negahdary[SUP] 1 [/SUP], Mario Hiroyuki Hirata[SUP] 2 [/SUP], Solange Kazumi Sakata[SUP] 3 [/SUP], Rozana Mesquita Ciconelli[SUP] 4 [/SUP], Gisele Medeiros Bastos[SUP] 4 [/SUP], Jéssica Bassani Borges[SUP] 4 [/SUP], Helena Strelow Thurow[SUP] 4 [/SUP], Alceu Totti Silveira Junior[SUP] 5 [/SUP], Marcelo Ferraz Sampaio[SUP] 4 [/SUP], Larissa Berretta Guimarães[SUP] 4 [/SUP], Bruno Sussumu Maeda[SUP] 4 [/SUP], Lúcio Angnes[SUP] 6 [/SUP]
Affiliations
Abstract
In this research, by using aptamer-conjugated gold nanoparticles (aptamer-AuNPs) and a modified glassy carbon electrode (GCE) with reduced graphene oxide (rGO) and Acropora-like gold (ALG) nanostructure, a sandwich-like system provided for sensitive detection of heat shock protein 70 kDa (HSP70), which applied as a functional biomarker in diagnosis/prognosis of COVID-19. Initially, the surface of the GCE was improved with rGO and ALG nanostructures, respectively. Then, an aptamer sequence as the first part of the bioreceptor was covalently bound on the surface of the GCE/rGO/ALG nanostructures. After adding the analyte, the second part of the bioreceptor (aptamer-AuNPs) was immobilized on the electrode surface to improve the diagnostic performance. The designed aptasensor detected HSP70 in a wide linear range, from 5 pg mL[SUP]-1[/SUP] to 75 ng mL[SUP]-1[/SUP], with a limit of detection (LOD) of ∼2 pg mL[SUP]-1[/SUP]. The aptasensor was stable for 3 weeks and applicable in detecting 40 real plasma samples of COVID-19 patients. The diagnostic sensitivity and specificity were 90% and 85%, respectively, compared with the reverse transcription-polymerase chain reaction (RT-PCR) method.
Keywords: Aptasensor; Bioelectrochemistry; Coronavirus disease 2019 (COVID-19); Gold nanomaterials; Heat shock protein 70 kDa (HSP70); Reduced graphene oxide (rGO).
. 2023 Feb 15;1242:340716.
doi: 10.1016/j.aca.2022.340716. Epub 2022 Dec 8.
Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70): Application in diagnosis/prognosis of coronavirus disease 2019 (COVID-19)
Masoud Negahdary[SUP] 1 [/SUP], Mario Hiroyuki Hirata[SUP] 2 [/SUP], Solange Kazumi Sakata[SUP] 3 [/SUP], Rozana Mesquita Ciconelli[SUP] 4 [/SUP], Gisele Medeiros Bastos[SUP] 4 [/SUP], Jéssica Bassani Borges[SUP] 4 [/SUP], Helena Strelow Thurow[SUP] 4 [/SUP], Alceu Totti Silveira Junior[SUP] 5 [/SUP], Marcelo Ferraz Sampaio[SUP] 4 [/SUP], Larissa Berretta Guimarães[SUP] 4 [/SUP], Bruno Sussumu Maeda[SUP] 4 [/SUP], Lúcio Angnes[SUP] 6 [/SUP]
Affiliations
- PMID: 36657883
- DOI: 10.1016/j.aca.2022.340716
Abstract
In this research, by using aptamer-conjugated gold nanoparticles (aptamer-AuNPs) and a modified glassy carbon electrode (GCE) with reduced graphene oxide (rGO) and Acropora-like gold (ALG) nanostructure, a sandwich-like system provided for sensitive detection of heat shock protein 70 kDa (HSP70), which applied as a functional biomarker in diagnosis/prognosis of COVID-19. Initially, the surface of the GCE was improved with rGO and ALG nanostructures, respectively. Then, an aptamer sequence as the first part of the bioreceptor was covalently bound on the surface of the GCE/rGO/ALG nanostructures. After adding the analyte, the second part of the bioreceptor (aptamer-AuNPs) was immobilized on the electrode surface to improve the diagnostic performance. The designed aptasensor detected HSP70 in a wide linear range, from 5 pg mL[SUP]-1[/SUP] to 75 ng mL[SUP]-1[/SUP], with a limit of detection (LOD) of ∼2 pg mL[SUP]-1[/SUP]. The aptasensor was stable for 3 weeks and applicable in detecting 40 real plasma samples of COVID-19 patients. The diagnostic sensitivity and specificity were 90% and 85%, respectively, compared with the reverse transcription-polymerase chain reaction (RT-PCR) method.
Keywords: Aptasensor; Bioelectrochemistry; Coronavirus disease 2019 (COVID-19); Gold nanomaterials; Heat shock protein 70 kDa (HSP70); Reduced graphene oxide (rGO).