tetano
Editor, Senior Moderator
Anal Chim Acta
. 2023 Jan 25;1239:340651.
doi: 10.1016/j.aca.2022.340651. Epub 2022 Nov 21.
Plasmonic nanostructure-enhanced Raman scattering for detection of SARS-CoV-2 nucleocapsid protein and spike protein variants
Yi-Jui Yeh[SUP] 1 [/SUP], Trong-Nghia Le[SUP] 2 [/SUP], Wesley Wei-Wen Hsiao[SUP] 3 [/SUP], Kuo-Lun Tung[SUP] 4 [/SUP], Kostya Ken Ostrikov[SUP] 5 [/SUP], Wei-Hung Chiang[SUP] 6 [/SUP]
Affiliations
Abstract
Epidemiological control and public health monitoring during the outbreaks of infectious viral diseases rely on the ability to detect viral pathogens. Here we demonstrate a rapid, sensitive, and selective nanotechnology-enhanced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection based on the surface-enhanced Raman scattering (SERS) responses from the plasma-engineered, variant-specific antibody-functionalized silver microplasma-engineered nanoassemblies (AgMEN) interacting with the SARS-CoV-2 spike (S) and nucleocapsid (N) proteins. The three-dimensional (3D) porous AgMEN with plasmonic-active nanostructures provide a high sensitivity to virus detection via the remarkable SERS signal collection. Moreover, the variant-specific antibody-functionalization on the SERS-active AgMEN enabled the high selectivity of the SARS-CoV-2 S variants, including wild-type, Alpha, Delta, and Omicron, under the simulated human saliva conditions. The exceptional ultrahigh sensitivity of our SERS biosensor was demonstrated via SARS-CoV-2 S and N proteins at the detection limit of 1 fg mL[SUP]-1[/SUP] and 0.1 pg mL[SUP]-1[/SUP], respectively. Our work demonstrates a versatile SERS-based detection platform can be applied for the ultrasensitive detection of virus variants, infectious diseases, and cancer biomarkers.
Keywords: Pathogen detection; Point-of-care test; Raman spectroscopy; Rapid diagnosis; SARS-CoV-2; SERS.
. 2023 Jan 25;1239:340651.
doi: 10.1016/j.aca.2022.340651. Epub 2022 Nov 21.
Plasmonic nanostructure-enhanced Raman scattering for detection of SARS-CoV-2 nucleocapsid protein and spike protein variants
Yi-Jui Yeh[SUP] 1 [/SUP], Trong-Nghia Le[SUP] 2 [/SUP], Wesley Wei-Wen Hsiao[SUP] 3 [/SUP], Kuo-Lun Tung[SUP] 4 [/SUP], Kostya Ken Ostrikov[SUP] 5 [/SUP], Wei-Hung Chiang[SUP] 6 [/SUP]
Affiliations
- PMID: 36628748
- DOI: 10.1016/j.aca.2022.340651
Abstract
Epidemiological control and public health monitoring during the outbreaks of infectious viral diseases rely on the ability to detect viral pathogens. Here we demonstrate a rapid, sensitive, and selective nanotechnology-enhanced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection based on the surface-enhanced Raman scattering (SERS) responses from the plasma-engineered, variant-specific antibody-functionalized silver microplasma-engineered nanoassemblies (AgMEN) interacting with the SARS-CoV-2 spike (S) and nucleocapsid (N) proteins. The three-dimensional (3D) porous AgMEN with plasmonic-active nanostructures provide a high sensitivity to virus detection via the remarkable SERS signal collection. Moreover, the variant-specific antibody-functionalization on the SERS-active AgMEN enabled the high selectivity of the SARS-CoV-2 S variants, including wild-type, Alpha, Delta, and Omicron, under the simulated human saliva conditions. The exceptional ultrahigh sensitivity of our SERS biosensor was demonstrated via SARS-CoV-2 S and N proteins at the detection limit of 1 fg mL[SUP]-1[/SUP] and 0.1 pg mL[SUP]-1[/SUP], respectively. Our work demonstrates a versatile SERS-based detection platform can be applied for the ultrasensitive detection of virus variants, infectious diseases, and cancer biomarkers.
Keywords: Pathogen detection; Point-of-care test; Raman spectroscopy; Rapid diagnosis; SARS-CoV-2; SERS.