tetano
Editor, Senior Moderator
Anal Chim Acta
. 2025 Jan 15:1335:343474.
doi: 10.1016/j.aca.2024.343474. Epub 2024 Nov 23. Nanozymes featuring a mesoporous silica shell for rapid enrichment and ultrasensitive lateral flow immunoassay of influenza A
Jing Liang[SUP] 1 [/SUP], Xiaoxian Liu[SUP] 2 [/SUP], Zhenzhen Liu[SUP] 2 [/SUP], Xuanming Xu[SUP] 3 [/SUP], Yinuo Sun[SUP] 2 [/SUP], Yuan Chen[SUP] 3 [/SUP], Rui Xiao[SUP] 4 [/SUP], Yajie Wang[SUP] 5 [/SUP]
Affiliations
Background: Respiratory illnesses stemming from influenza A viruses represent a significant worldwide health concern. There is an immediate need for a rapid and sensitive method to detect influenza A viruses early, without requiring extra equipment.
Results: Here, we established a lateral flow immunoassay (LFIA) for the detection of influenza A (Flu A) using a "three-in-one" multifunctional mesoporous Fe[SUB]3[/SUB]O[SUB]4[/SUB]@SiO[SUB]2[/SUB]@Pt nanozymes (Fe[SUB]3[/SUB]O[SUB]4[/SUB]@MSiO[SUB]2[/SUB]@Pt NZs) with excellent magnetic separation properties, colorimetric, and peroxidase-like (POD-like) activities. Effective enrichment of target Flu A in complex samples as well as greater loading of Pt particles by mesoporous structures with large specific surface area to enhance POD-like activity can significantly improve the detection sensitivity of the LFIA. After colorimetric amplification by Fe[SUB]3[/SUB]O[SUB]4[/SUB]@MSiO[SUB]2[/SUB]@Pt tags catalysis, the qualitative and quantitative results of detection for Flu A nucleoprotein (Flu A-NP) were 0.01 and 0.0089 ng mL[SUP]-1[/SUP], respectively. This indicated a sensitivity approximately 100 times greater than commercially available colloidal Au nanoparticle (AuNP)-based LFIA strips. For detection of inactivated H1N1 virus, quantification can be as low as 33 copies mL[SUP]-1[/SUP]. Moreover, it demonstrated high accuracy in pharyngeal swab sample simulation experiments.
Significance: Therefore, the proposed platform based on Fe[SUB]3[/SUB]O[SUB]4[/SUB]@MSiO[SUB]2[/SUB]@Pt NZs-LFIA offered a promising approach for point-of-care testing (POCT), enabling rapid and ultrasensitive diagnosis of Flu A.
Keywords: Colorimetric; Composite nanozymes; Influenza A; Lateral flow immunoassay; Mesoporous material.
. 2025 Jan 15:1335:343474.
doi: 10.1016/j.aca.2024.343474. Epub 2024 Nov 23. Nanozymes featuring a mesoporous silica shell for rapid enrichment and ultrasensitive lateral flow immunoassay of influenza A
Jing Liang[SUP] 1 [/SUP], Xiaoxian Liu[SUP] 2 [/SUP], Zhenzhen Liu[SUP] 2 [/SUP], Xuanming Xu[SUP] 3 [/SUP], Yinuo Sun[SUP] 2 [/SUP], Yuan Chen[SUP] 3 [/SUP], Rui Xiao[SUP] 4 [/SUP], Yajie Wang[SUP] 5 [/SUP]
Affiliations
- PMID: 39643324
- DOI: 10.1016/j.aca.2024.343474
Background: Respiratory illnesses stemming from influenza A viruses represent a significant worldwide health concern. There is an immediate need for a rapid and sensitive method to detect influenza A viruses early, without requiring extra equipment.
Results: Here, we established a lateral flow immunoassay (LFIA) for the detection of influenza A (Flu A) using a "three-in-one" multifunctional mesoporous Fe[SUB]3[/SUB]O[SUB]4[/SUB]@SiO[SUB]2[/SUB]@Pt nanozymes (Fe[SUB]3[/SUB]O[SUB]4[/SUB]@MSiO[SUB]2[/SUB]@Pt NZs) with excellent magnetic separation properties, colorimetric, and peroxidase-like (POD-like) activities. Effective enrichment of target Flu A in complex samples as well as greater loading of Pt particles by mesoporous structures with large specific surface area to enhance POD-like activity can significantly improve the detection sensitivity of the LFIA. After colorimetric amplification by Fe[SUB]3[/SUB]O[SUB]4[/SUB]@MSiO[SUB]2[/SUB]@Pt tags catalysis, the qualitative and quantitative results of detection for Flu A nucleoprotein (Flu A-NP) were 0.01 and 0.0089 ng mL[SUP]-1[/SUP], respectively. This indicated a sensitivity approximately 100 times greater than commercially available colloidal Au nanoparticle (AuNP)-based LFIA strips. For detection of inactivated H1N1 virus, quantification can be as low as 33 copies mL[SUP]-1[/SUP]. Moreover, it demonstrated high accuracy in pharyngeal swab sample simulation experiments.
Significance: Therefore, the proposed platform based on Fe[SUB]3[/SUB]O[SUB]4[/SUB]@MSiO[SUB]2[/SUB]@Pt NZs-LFIA offered a promising approach for point-of-care testing (POCT), enabling rapid and ultrasensitive diagnosis of Flu A.
Keywords: Colorimetric; Composite nanozymes; Influenza A; Lateral flow immunoassay; Mesoporous material.