tetano
Editor, Senior Moderator
Nanomedicine. 2016 Apr 22. pii: S1549-9634(16)30033-8. doi: 10.1016/j.nano.2016.04.005. [Epub ahead of print]
[h=1]An oil-in-water nanoemulsion enhances immunogenicity of H5N1 vaccine in mice.[/h] Cao W[SUP]1[/SUP], Davis WG[SUP]2[/SUP], Kim JH[SUP]2[/SUP], De La Cruz JA[SUP]2[/SUP], Taylor A[SUP]2[/SUP], Hendrickson GR[SUP]3[/SUP], Kumar A[SUP]2[/SUP], Ranjan P[SUP]2[/SUP], Lyon LA[SUP]3[/SUP], Katz JM[SUP]2[/SUP], Gangappa S[SUP]2[/SUP], Sambhara S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] To enhance the immunogenicity of the Influenza H5N1 vaccine, we developed an oil-in-water nanoemulsion (NE) adjuvant. NE displayed good temperature stability and maintained particle size. More importantly, it significantly enhanced IL-6 and MCP-1 production to recruit innate cells, including neutrophils, monocytes/macrophages and dendritic cells to the local environment. Furthermore, NE enhanced dendritic cell function to induce robust antigen-specific T and B cell immune responses. NE-adjuvanted H5N1 vaccine not only elicited significantly higher and long-lasting antibody responses, but also conferred enhanced protection against homologous clade 1 as well as heterologous clade 2 H5N1 virus challenge in young as well as in aged mice. The pre-existing immunity to seasonal influenza did not affect the immunogenicity of NE-adjuvanted H5N1 vaccine.
Copyright ? 2015. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] H5N1 vaccine; adaptive immunity; adjuvant; influenza; innate immunity; oil-in-water nanoemulsion
PMID: 27112307 [PubMed - as supplied by publisher]
[h=1]An oil-in-water nanoemulsion enhances immunogenicity of H5N1 vaccine in mice.[/h] Cao W[SUP]1[/SUP], Davis WG[SUP]2[/SUP], Kim JH[SUP]2[/SUP], De La Cruz JA[SUP]2[/SUP], Taylor A[SUP]2[/SUP], Hendrickson GR[SUP]3[/SUP], Kumar A[SUP]2[/SUP], Ranjan P[SUP]2[/SUP], Lyon LA[SUP]3[/SUP], Katz JM[SUP]2[/SUP], Gangappa S[SUP]2[/SUP], Sambhara S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] To enhance the immunogenicity of the Influenza H5N1 vaccine, we developed an oil-in-water nanoemulsion (NE) adjuvant. NE displayed good temperature stability and maintained particle size. More importantly, it significantly enhanced IL-6 and MCP-1 production to recruit innate cells, including neutrophils, monocytes/macrophages and dendritic cells to the local environment. Furthermore, NE enhanced dendritic cell function to induce robust antigen-specific T and B cell immune responses. NE-adjuvanted H5N1 vaccine not only elicited significantly higher and long-lasting antibody responses, but also conferred enhanced protection against homologous clade 1 as well as heterologous clade 2 H5N1 virus challenge in young as well as in aged mice. The pre-existing immunity to seasonal influenza did not affect the immunogenicity of NE-adjuvanted H5N1 vaccine.
Copyright ? 2015. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] H5N1 vaccine; adaptive immunity; adjuvant; influenza; innate immunity; oil-in-water nanoemulsion
PMID: 27112307 [PubMed - as supplied by publisher]