tetano
Editor, Senior Moderator
Arch Virol. 2013 Oct 19. [Epub ahead of print]
An NA-deficient 2009 pandemic H1N1 influenza virus mutant can efficiently replicate in cultured cells.
Inoue E, Ieko M, Takahashi N, Osawa Y, Okazaki K.
Source
Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
Abstract
We identified a novel neuraminidase (NA)-deficient virus that was a 2009 pandemic influenza H1N1 virus mutant. The mutant virus had a deletion of 1,009 nt in the NA gene and lacked an enzymatic domain. Although the yield of the NA-deficient virus was limited, it formed large plaques when applied to MDCK cell cultures, indicating that the virus was able to spread to adjacent cells. Furthermore, the NA-deficient virus was eluted from chicken erythrocytes at 37 ?C, even in the presence of the antiviral drug peramivir. Spread of this NA-deficient virus may pose a potential threat to anti-influenza therapies.
PMID:
24142272
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24142272
An NA-deficient 2009 pandemic H1N1 influenza virus mutant can efficiently replicate in cultured cells.
Inoue E, Ieko M, Takahashi N, Osawa Y, Okazaki K.
Source
Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
Abstract
We identified a novel neuraminidase (NA)-deficient virus that was a 2009 pandemic influenza H1N1 virus mutant. The mutant virus had a deletion of 1,009 nt in the NA gene and lacked an enzymatic domain. Although the yield of the NA-deficient virus was limited, it formed large plaques when applied to MDCK cell cultures, indicating that the virus was able to spread to adjacent cells. Furthermore, the NA-deficient virus was eluted from chicken erythrocytes at 37 ?C, even in the presence of the antiviral drug peramivir. Spread of this NA-deficient virus may pose a potential threat to anti-influenza therapies.
PMID:
24142272
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24142272