tetano
Editor, Senior Moderator
Sci Rep. 2016 Oct 31;6:36015. doi: 10.1038/srep36015.
[h=1]An extract from Taxodium distichum targets hemagglutinin- and neuraminidase-related activities of influenza virus in vitro.[/h] Hsieh CF[SUP]1[/SUP], Chen YL[SUP]2[/SUP], Lin CF[SUP]3,[/SUP][SUP]4[/SUP], Ho JY[SUP]1[/SUP], Huang CH[SUP]3,[/SUP][SUP]4[/SUP], Chiu CH[SUP]5[/SUP], Hsieh PW[SUP]2,[/SUP][SUP]4,[/SUP][SUP]6,[/SUP][SUP]7[/SUP], Horng JT[SUP]1,[/SUP][SUP]4,[/SUP][SUP]5,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza virus remains an emerging virus and causes pandemics with high levels of fatality. After screening different plant extracts with potential anti-influenza activity, a water extract of Taxodium distichum stems (TDSWex) showed excellent activity against influenza viruses. The EC[SUB]50[/SUB] of TDSWex was 0.051 ? 0.024 mg/mL against influenza virus A/WSN/33. TDSWex had excellent antiviral efficacy against various strains of human influenza A and B viruses, particularly oseltamivir-resistant clinical isolates and a swine-origin influenza strain. We observed that the synthesis of viral RNA and protein were inhibited in the presence of TDSWex. The results of the time-of-addition assay suggested that TDSWex inhibited viral entry and budding. In the hemagglutination inhibition assay, TDSWex inhibited the hemagglutination of red blood cells, implying that the extract targeted hemagglutin-related functions such as viral entry. In the attachment and penetration assay, TDSWex showed antiviral activity with EC[SUB]50[/SUB]s of 0.045 ? 0.026 and 0.012 ? 0.003 mg/mL, respectively. In addition, TDSWex blocked neuraminidase activity. We conclude that TDSWex has bimodal activities against both hemagglutinin and neuraminidase during viral replication.
PMID: 27796330 DOI: 10.1038/srep36015
[PubMed - in process] Free full text
[h=1]An extract from Taxodium distichum targets hemagglutinin- and neuraminidase-related activities of influenza virus in vitro.[/h] Hsieh CF[SUP]1[/SUP], Chen YL[SUP]2[/SUP], Lin CF[SUP]3,[/SUP][SUP]4[/SUP], Ho JY[SUP]1[/SUP], Huang CH[SUP]3,[/SUP][SUP]4[/SUP], Chiu CH[SUP]5[/SUP], Hsieh PW[SUP]2,[/SUP][SUP]4,[/SUP][SUP]6,[/SUP][SUP]7[/SUP], Horng JT[SUP]1,[/SUP][SUP]4,[/SUP][SUP]5,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza virus remains an emerging virus and causes pandemics with high levels of fatality. After screening different plant extracts with potential anti-influenza activity, a water extract of Taxodium distichum stems (TDSWex) showed excellent activity against influenza viruses. The EC[SUB]50[/SUB] of TDSWex was 0.051 ? 0.024 mg/mL against influenza virus A/WSN/33. TDSWex had excellent antiviral efficacy against various strains of human influenza A and B viruses, particularly oseltamivir-resistant clinical isolates and a swine-origin influenza strain. We observed that the synthesis of viral RNA and protein were inhibited in the presence of TDSWex. The results of the time-of-addition assay suggested that TDSWex inhibited viral entry and budding. In the hemagglutination inhibition assay, TDSWex inhibited the hemagglutination of red blood cells, implying that the extract targeted hemagglutin-related functions such as viral entry. In the attachment and penetration assay, TDSWex showed antiviral activity with EC[SUB]50[/SUB]s of 0.045 ? 0.026 and 0.012 ? 0.003 mg/mL, respectively. In addition, TDSWex blocked neuraminidase activity. We conclude that TDSWex has bimodal activities against both hemagglutinin and neuraminidase during viral replication.
PMID: 27796330 DOI: 10.1038/srep36015
[PubMed - in process] Free full text