tetano
Editor, Senior Moderator
Biochem Biophys Res Commun. 2019 Aug 14. pii: S0006-291X(19)31572-4. doi: 10.1016/j.bbrc.2019.08.055. [Epub ahead of print]
[h=1]An epitope on the stem region of hemagglutinin of H1N1 influenza A virus recognized by neutralizing monoclonal antibody.[/h] Yan L[SUP]1[/SUP], Wang H[SUP]2[/SUP], Sun L[SUP]1[/SUP], Liu Y[SUP]1[/SUP], Sun J[SUP]1[/SUP], Zhao X[SUP]1[/SUP], Li Y[SUP]1[/SUP], Xie X[SUP]3[/SUP], Hu J[SUP]4[/SUP].
[h=3]Author information[/h] 1 Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, China; Key Laboratory of Infection and Immunity Disease of Shaanxi Province, Xi'an, 710068, China. 2 Institute of Integrated Medicine, Shaanxi University of Chinese Medicine, Xi'an, 712046, China. 3 Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, 710069, China; Department of Translational Medicine, Institute of Integrated Medical Information, Xi'an, 710016, China. Electronic address: xiexin@nwu.edu.cn. 4 Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, China; Key Laboratory of Infection and Immunity Disease of Shaanxi Province, Xi'an, 710068, China. Electronic address: submit0917@126.com.
[h=3]Abstract[/h] Influenza A viruses are a major threat to human health and inflict a significant public health challenge worldwide. Hemagglutinin (HA) is the main contributor to the infectivity of the virus and is a potential target for the development of antiviral therapeutic agents. The stem region of HA, which has been shown to be conserved, is the primary target in the development of antibody-based therapeutic strategies and preventive tools. Here, we confirmed the neutralizing activity and prophylactic efficacy of murine monoclonal antibody PR8-25 as well as Western blot analysis of different influenza virus strains. Then, we identified the epitope of PR8-25, 328-LRMVTGLRNIPS-339, by phage-displayed 12-mer random peptide library. The identified epitope targeted in the stem region of HA, specifically at the C-terminal of the HA1 fragment. This result suggest that the identified epitope may be a potential basis for antiviral drugs and stem-based universal influenza vaccines.
Copyright ? 2019 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Epitope peptide; Hemagglutinin; Influenza virus; Neutralizing antibodies; Vaccine
PMID: 31421820 DOI: 10.1016/j.bbrc.2019.08.055
[h=1]An epitope on the stem region of hemagglutinin of H1N1 influenza A virus recognized by neutralizing monoclonal antibody.[/h] Yan L[SUP]1[/SUP], Wang H[SUP]2[/SUP], Sun L[SUP]1[/SUP], Liu Y[SUP]1[/SUP], Sun J[SUP]1[/SUP], Zhao X[SUP]1[/SUP], Li Y[SUP]1[/SUP], Xie X[SUP]3[/SUP], Hu J[SUP]4[/SUP].
[h=3]Author information[/h] 1 Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, China; Key Laboratory of Infection and Immunity Disease of Shaanxi Province, Xi'an, 710068, China. 2 Institute of Integrated Medicine, Shaanxi University of Chinese Medicine, Xi'an, 712046, China. 3 Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, 710069, China; Department of Translational Medicine, Institute of Integrated Medical Information, Xi'an, 710016, China. Electronic address: xiexin@nwu.edu.cn. 4 Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, China; Key Laboratory of Infection and Immunity Disease of Shaanxi Province, Xi'an, 710068, China. Electronic address: submit0917@126.com.
[h=3]Abstract[/h] Influenza A viruses are a major threat to human health and inflict a significant public health challenge worldwide. Hemagglutinin (HA) is the main contributor to the infectivity of the virus and is a potential target for the development of antiviral therapeutic agents. The stem region of HA, which has been shown to be conserved, is the primary target in the development of antibody-based therapeutic strategies and preventive tools. Here, we confirmed the neutralizing activity and prophylactic efficacy of murine monoclonal antibody PR8-25 as well as Western blot analysis of different influenza virus strains. Then, we identified the epitope of PR8-25, 328-LRMVTGLRNIPS-339, by phage-displayed 12-mer random peptide library. The identified epitope targeted in the stem region of HA, specifically at the C-terminal of the HA1 fragment. This result suggest that the identified epitope may be a potential basis for antiviral drugs and stem-based universal influenza vaccines.
Copyright ? 2019 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Epitope peptide; Hemagglutinin; Influenza virus; Neutralizing antibodies; Vaccine
PMID: 31421820 DOI: 10.1016/j.bbrc.2019.08.055