tetano
Editor, Senior Moderator
Anal Chem. 2015 Sep 11. [Epub ahead of print]
[h=1]An electrochemical label-free and reagentless genosensor based on an ion barrier "switch off system" for DNA sequence-specific detection of the Avian influenza virus.[/h] Kurzątkowska K, Sirko A, Zag?rski-Ostoja W, Dehaen W, Radecka H, Radecki J.
[h=3]Abstract[/h] This paper concerns the development of genosensors based on redox active monolayers incorporating (dipyrromethene)2Cu(II) and (dipyrromethene)2Co(II) complexes formed step by step on the gold electrode surface. They were applied for electrochemical determination of oligonucleotide sequences related to the avian influenza virus (AIV) type H5N1. A 20-mer probe (NH2-NC3) was covalently attached to the gold electrode surface via a reaction performed in the presence of EDC/NHS between the amine group present in the probe and carboxylic groups present on the surface of the redox active layer. Each modification step has been controlled with Osteryoung square-wave voltammetry. The genosensor incorporating the (dipyrromethene)2Cu(II) complex was able to detect a fully complementary ssDNA target with a detection limit of 1.39 pM. A linear dynamic range was observed from 1 pM to 10 pM. This genosensor displays good discrimination between three ssDNA targets studied: either fully complementary, partially complementary (with only six complementary bases) and totally not complementary to the probe. When the (dipyrromethene)2Co(II) complex was applied, a detection limit of 1.28 pM for the fully complementary target was obtained. However, this genosensor was not able to discriminate partially complementary and totally not complementary oligonucleotide sequences to the probe. Electrochemical measurements, using both types of genosensors in the presence of different supporting electrolytes were performed in order to elaborate a new mechanism of analytical signal generation based on an ion barrier "switch off system".
PMID: 26359972 [PubMed - as supplied by publisher]
[h=1]An electrochemical label-free and reagentless genosensor based on an ion barrier "switch off system" for DNA sequence-specific detection of the Avian influenza virus.[/h] Kurzątkowska K, Sirko A, Zag?rski-Ostoja W, Dehaen W, Radecka H, Radecki J.
[h=3]Abstract[/h] This paper concerns the development of genosensors based on redox active monolayers incorporating (dipyrromethene)2Cu(II) and (dipyrromethene)2Co(II) complexes formed step by step on the gold electrode surface. They were applied for electrochemical determination of oligonucleotide sequences related to the avian influenza virus (AIV) type H5N1. A 20-mer probe (NH2-NC3) was covalently attached to the gold electrode surface via a reaction performed in the presence of EDC/NHS between the amine group present in the probe and carboxylic groups present on the surface of the redox active layer. Each modification step has been controlled with Osteryoung square-wave voltammetry. The genosensor incorporating the (dipyrromethene)2Cu(II) complex was able to detect a fully complementary ssDNA target with a detection limit of 1.39 pM. A linear dynamic range was observed from 1 pM to 10 pM. This genosensor displays good discrimination between three ssDNA targets studied: either fully complementary, partially complementary (with only six complementary bases) and totally not complementary to the probe. When the (dipyrromethene)2Co(II) complex was applied, a detection limit of 1.28 pM for the fully complementary target was obtained. However, this genosensor was not able to discriminate partially complementary and totally not complementary oligonucleotide sequences to the probe. Electrochemical measurements, using both types of genosensors in the presence of different supporting electrolytes were performed in order to elaborate a new mechanism of analytical signal generation based on an ion barrier "switch off system".
PMID: 26359972 [PubMed - as supplied by publisher]