tetano
Editor, Senior Moderator
Res Vet Sci. 2020 Mar 10;130:203-206. doi: 10.1016/j.rvsc.2020.03.017. [Epub ahead of print]
Amino acid substitutions involved in the adaptation of a novel H7N7 avian influenza virus in mice.
Wu H[SUP]1[/SUP], Liu F[SUP]2[/SUP], Yang F[SUP]2[/SUP], Xiao Y[SUP]2[/SUP], Yao H[SUP]2[/SUP], Wu N[SUP]3[/SUP].
Author information
Abstract
The H7N7 avian influenza viruses can infect humans and poses a great threat to human health. To identify the amino acid substitutions that are associated with adaptation of avian-origin H7N7 virus to mammals, adaptation of the H7N7 virus was carried out by serial lung-to-lung passage in mice. Genomic analysis of the mouse-adapted virus revealed amino acid changes in the PB2 (E525G, M645I, and D701N), NP (I475V), HA(D103N), and NA(K142E) proteins. The adapted H7N7 virus was more virulent in mice than the wild-type virus. Our results suggest that continued surveillance of poultry populations for these substitutions in the H7N7 virus is required.
Copyright ? 2020. Published by Elsevier Ltd.
KEYWORDS:
Genetic variability; H7N7 avian influenza virus; Mammals; Substitutions; Virulence; Zoonotic potential
PMID:32200160DOI:10.1016/j.rvsc.2020.03.017
Amino acid substitutions involved in the adaptation of a novel H7N7 avian influenza virus in mice.
Wu H[SUP]1[/SUP], Liu F[SUP]2[/SUP], Yang F[SUP]2[/SUP], Xiao Y[SUP]2[/SUP], Yao H[SUP]2[/SUP], Wu N[SUP]3[/SUP].
Author information
Abstract
The H7N7 avian influenza viruses can infect humans and poses a great threat to human health. To identify the amino acid substitutions that are associated with adaptation of avian-origin H7N7 virus to mammals, adaptation of the H7N7 virus was carried out by serial lung-to-lung passage in mice. Genomic analysis of the mouse-adapted virus revealed amino acid changes in the PB2 (E525G, M645I, and D701N), NP (I475V), HA(D103N), and NA(K142E) proteins. The adapted H7N7 virus was more virulent in mice than the wild-type virus. Our results suggest that continued surveillance of poultry populations for these substitutions in the H7N7 virus is required.
Copyright ? 2020. Published by Elsevier Ltd.
KEYWORDS:
Genetic variability; H7N7 avian influenza virus; Mammals; Substitutions; Virulence; Zoonotic potential
PMID:32200160DOI:10.1016/j.rvsc.2020.03.017