tetano
Editor, Senior Moderator
J Virol. 2011 Oct 19. [Epub ahead of print]
Amino acid changes in hemagglutinin contribute to the replication of oseltamivir-resistant H1N1 influenza viruses.
Ginting TE, Shinya K, Kyan Y, Makino A, Matsumoto N, Kaneda S, Kawaoka Y.
Source
Center for Infectious Diseases, Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo, Japan.
Abstract
Oseltamivir-resistant H1N1 influenza viruses emerged in 2007-2008 and have subsequently circulated widely. However, prior to 2007-2008, viruses possessing the neuraminidase (NA) H274Y mutation, which confers oseltamivir resistance, generally had low growth capability. NA mutations that compensate for the deleterious effect of the NA H274Y mutation have since been identified. Given the importance of the functional balance between hemagglutinin (HA) and NA, we focused on amino acid changes in HA. Reverse genetics analysis showed that a mutation at residue 82, 141, or 189 of the HA protein promotes virus replication in the presence of the NA H274Y mutation. Our findings thus identify HA mutations that contributed to the replacement of the oseltamivir-sensitive viruses of 2007-2008.
PMID:
22013054
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22013054
Amino acid changes in hemagglutinin contribute to the replication of oseltamivir-resistant H1N1 influenza viruses.
Ginting TE, Shinya K, Kyan Y, Makino A, Matsumoto N, Kaneda S, Kawaoka Y.
Source
Center for Infectious Diseases, Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo, Japan.
Abstract
Oseltamivir-resistant H1N1 influenza viruses emerged in 2007-2008 and have subsequently circulated widely. However, prior to 2007-2008, viruses possessing the neuraminidase (NA) H274Y mutation, which confers oseltamivir resistance, generally had low growth capability. NA mutations that compensate for the deleterious effect of the NA H274Y mutation have since been identified. Given the importance of the functional balance between hemagglutinin (HA) and NA, we focused on amino acid changes in HA. Reverse genetics analysis showed that a mutation at residue 82, 141, or 189 of the HA protein promotes virus replication in the presence of the NA H274Y mutation. Our findings thus identify HA mutations that contributed to the replacement of the oseltamivir-sensitive viruses of 2007-2008.
PMID:
22013054
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22013054