tetano
Editor, Senior Moderator
Am J Reprod Immunol
. 2022 Sep 19.
doi: 10.1111/aji.13626. Online ahead of print.
The immunological role of b7-h4 in pregnant women with sars-cov2 infection
Liyan Duan[SUP] 1 [/SUP], Beatrix Reisch[SUP] 1 [/SUP], Pawel Mach[SUP] 1 [/SUP], Rainer Kimmig[SUP] 1 [/SUP], Alexandra Gellhaus[SUP] 1 [/SUP], Antonella Iannaccone[SUP] 1 [/SUP]
Affiliations
Abstract
Problem: T-cells are key players in fighting the coronavirus disease 2019 (COVID-19). The checkpoint molecule B7-H4, a member of the B7 family, can inhibit T-cell activation and proliferation by inhibiting NF-kb expression. We aimed to elucidate the immunological role of soluble B7-H4 (sB7-H4) and B7-H4 in pregnant women suffered from an acute Sars-Cov2 infection.
Methods: Expression levels of sB7-H4 and cytokines were detected by enzyme linked immunosorbent assay. B7-H4 and cytokines mRNA expression was analyzed by qPCR, and B7-H4 and NF-κb (p65) protein levels were investigated by western blot and immunofluorescence staining in placenta chorionic villous and decidual basalis tissues of COVID-19 affected women and healthy controls.
Results: Fibrinoid necrosis in the periphery of placental villi was increased in the COVID-19-affected patients. sB7-H4 protein in maternal and cord blood serum and IL-6 / IL-10 were increased while leukocyte level was were decreased during SARS-CoV-2 infection. Serum sB7-H4 level was increased with severity of SARS-Cov-2 infection. Cytokines (IL-6, IL-18, IL-1β, TNF-α), B7-H4 mRNA and protein in the decidual basalis tissues of COVID-19-infected pregnant women were significantly increased compared to healthy controls. IL-18 and IL-1β were significantly increased in the placenta chorionic villous samples of COVID-19 affected patients, while NF-κb (p65) expression was decreased.
Conclusions: The expression of the immunological marker sB7-H4 correlated with the severity of COVID-19 disease in pregnant women. sB7-H4 and B7-H4 can be used to monitor the progression of COVID-19 infection during pregnancy, and for evaluating of the maternal immune status. This article is protected by copyright. All rights reserved.
Keywords: B7-H4; SARS-CoV-2 T-cells; immune regulation; infection; pregnancy.
. 2022 Sep 19.
doi: 10.1111/aji.13626. Online ahead of print.
The immunological role of b7-h4 in pregnant women with sars-cov2 infection
Liyan Duan[SUP] 1 [/SUP], Beatrix Reisch[SUP] 1 [/SUP], Pawel Mach[SUP] 1 [/SUP], Rainer Kimmig[SUP] 1 [/SUP], Alexandra Gellhaus[SUP] 1 [/SUP], Antonella Iannaccone[SUP] 1 [/SUP]
Affiliations
- PMID: 36121927
- DOI: 10.1111/aji.13626
Abstract
Problem: T-cells are key players in fighting the coronavirus disease 2019 (COVID-19). The checkpoint molecule B7-H4, a member of the B7 family, can inhibit T-cell activation and proliferation by inhibiting NF-kb expression. We aimed to elucidate the immunological role of soluble B7-H4 (sB7-H4) and B7-H4 in pregnant women suffered from an acute Sars-Cov2 infection.
Methods: Expression levels of sB7-H4 and cytokines were detected by enzyme linked immunosorbent assay. B7-H4 and cytokines mRNA expression was analyzed by qPCR, and B7-H4 and NF-κb (p65) protein levels were investigated by western blot and immunofluorescence staining in placenta chorionic villous and decidual basalis tissues of COVID-19 affected women and healthy controls.
Results: Fibrinoid necrosis in the periphery of placental villi was increased in the COVID-19-affected patients. sB7-H4 protein in maternal and cord blood serum and IL-6 / IL-10 were increased while leukocyte level was were decreased during SARS-CoV-2 infection. Serum sB7-H4 level was increased with severity of SARS-Cov-2 infection. Cytokines (IL-6, IL-18, IL-1β, TNF-α), B7-H4 mRNA and protein in the decidual basalis tissues of COVID-19-infected pregnant women were significantly increased compared to healthy controls. IL-18 and IL-1β were significantly increased in the placenta chorionic villous samples of COVID-19 affected patients, while NF-κb (p65) expression was decreased.
Conclusions: The expression of the immunological marker sB7-H4 correlated with the severity of COVID-19 disease in pregnant women. sB7-H4 and B7-H4 can be used to monitor the progression of COVID-19 infection during pregnancy, and for evaluating of the maternal immune status. This article is protected by copyright. All rights reserved.
Keywords: B7-H4; SARS-CoV-2 T-cells; immune regulation; infection; pregnancy.