tetano
Editor, Senior Moderator
Aging (Albany NY)
. 2021 Dec 12;13(undefined).
doi: 10.18632/aging.203761. Online ahead of print.
Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
Hsing-Yi Chung[SUP] 1 [/SUP], Ming-Jr Jian[SUP] 1 [/SUP], Chih-Kai Chang[SUP] 1 [/SUP], Jung-Chung Lin[SUP] 2 [/SUP], Kuo-Ming Yeh[SUP] 2 [/SUP], Ya-Sung Yang[SUP] 2 [/SUP], Chien-Wen Chen[SUP] 3 [/SUP], Shan-Shan Hsieh[SUP] 1 [/SUP], Sheng-Hui Tang[SUP] 1 [/SUP], Cherng-Lih Perng[SUP] 1 [/SUP], Feng-Yee Chang[SUP] 2 [/SUP], Kuo-Sheng Hung[SUP] 4 [/SUP], En-Sung Chen[SUP] 5 [/SUP], Mei-Hsiu Yang[SUP] 5 [/SUP], Hung-Sheng Shang[SUP] 1 [/SUP]
Affiliations
Abstract
Since the Coronavirus 19 (COVID-19) pandemic, several SARS-CoV-2 variants of concern (SARS-CoV-2 VOC) have been reported. The B.1.1.7 variant has been associated with increased mortality and transmission risk. Furthermore, cluster and possible co-infection cases could occur in the next influenza season or COVID-19 pandemic wave, warranting efficient diagnosis and treatment decision making. Here, we aimed to detect SARS-CoV-2 and other common respiratory viruses using multiplex RT-PCR developed on the LabTurbo AIO 48 open system. We performed a multicenter study to evaluate the performance and analytical sensitivity of the LabTurbo AIO 48 system for SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) using 652 nasopharyngeal swab clinical samples from patients. The LabTurbo AIO 48 system demonstrated a sensitivity of 9.4 copies/per PCR for N2 of SARS-CoV-2; 24 copies/per PCR for M of influenza A and B; and 24 copies/per PCR for N of RSV. The assay presented consistent performance in the multicenter study. The multiplex RT-PCR applied on the LabTurbo AIO 48 open platform provided highly sensitive, robust, and accurate results and enabled high-throughput detection of B.1.1.7, influenza A/B, and RSV with short turnaround times. Therefore, this automated molecular diagnostic assay could enable streamlined testing if COVID-19 becomes a seasonal disease.
Keywords: B.1.1.7 variant; COVID-19; SARS-CoV-2; SARS-CoV-2 VOC; multiplex testing.
. 2021 Dec 12;13(undefined).
doi: 10.18632/aging.203761. Online ahead of print.
Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
Hsing-Yi Chung[SUP] 1 [/SUP], Ming-Jr Jian[SUP] 1 [/SUP], Chih-Kai Chang[SUP] 1 [/SUP], Jung-Chung Lin[SUP] 2 [/SUP], Kuo-Ming Yeh[SUP] 2 [/SUP], Ya-Sung Yang[SUP] 2 [/SUP], Chien-Wen Chen[SUP] 3 [/SUP], Shan-Shan Hsieh[SUP] 1 [/SUP], Sheng-Hui Tang[SUP] 1 [/SUP], Cherng-Lih Perng[SUP] 1 [/SUP], Feng-Yee Chang[SUP] 2 [/SUP], Kuo-Sheng Hung[SUP] 4 [/SUP], En-Sung Chen[SUP] 5 [/SUP], Mei-Hsiu Yang[SUP] 5 [/SUP], Hung-Sheng Shang[SUP] 1 [/SUP]
Affiliations
- PMID: 34897035
- DOI: 10.18632/aging.203761
Abstract
Since the Coronavirus 19 (COVID-19) pandemic, several SARS-CoV-2 variants of concern (SARS-CoV-2 VOC) have been reported. The B.1.1.7 variant has been associated with increased mortality and transmission risk. Furthermore, cluster and possible co-infection cases could occur in the next influenza season or COVID-19 pandemic wave, warranting efficient diagnosis and treatment decision making. Here, we aimed to detect SARS-CoV-2 and other common respiratory viruses using multiplex RT-PCR developed on the LabTurbo AIO 48 open system. We performed a multicenter study to evaluate the performance and analytical sensitivity of the LabTurbo AIO 48 system for SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) using 652 nasopharyngeal swab clinical samples from patients. The LabTurbo AIO 48 system demonstrated a sensitivity of 9.4 copies/per PCR for N2 of SARS-CoV-2; 24 copies/per PCR for M of influenza A and B; and 24 copies/per PCR for N of RSV. The assay presented consistent performance in the multicenter study. The multiplex RT-PCR applied on the LabTurbo AIO 48 open platform provided highly sensitive, robust, and accurate results and enabled high-throughput detection of B.1.1.7, influenza A/B, and RSV with short turnaround times. Therefore, this automated molecular diagnostic assay could enable streamlined testing if COVID-19 becomes a seasonal disease.
Keywords: B.1.1.7 variant; COVID-19; SARS-CoV-2; SARS-CoV-2 VOC; multiplex testing.