tetano
Editor, Senior Moderator
Colloids Surf B Biointerfaces. 2011 Dec 31. [Epub ahead of print]
Aggregation effects of gold nanoparticles for single-base mismatch detection in influenza A (H1N1) DNA sequences using fluorescence and Raman measurements.
Ganbold EO, Kang T, Lee K, Lee SY, Joo SW.
Source
Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea.
Abstract
Aggregation effects of gold nanoparticles (AuNPs) were examined for the discrimination of single point mutations through the hybridization of oligonucleotides (25-50nM) modified with a fluorescent Texas red dye. The sequences of oligonucleotides were designed to detect the H1N1 virus gene. Single-base mismatch detection due to different adsorption propensities of oligonucleotides could be achieved using fluorescence quenching and surface-enhanced Raman scattering (SERS) properties of the dye. We observed that the addition of perfectly matched double stranded DNA (pmdsDNA), modified with the Texas red dye in the suspension of citrate-reduced AuNPs could increase fluorescence recovery intensities more substantially than either single-base mismatched double stranded DNA (sbmdsDNA) or single stranded DNA (ssDNA). We also tested DNA hybridization under both aggregation and near non-aggregation conditions for fluorescence measurements. A spectral difference in fluorescence intensity between pmdsDNA and sbmdsDNA appeared to be more discriminating under near non-aggregation than aggregation conditions. On the other hand, the SERS intensities of pmdsDNA and sbmdsDNA decreased more significantly than that of ssDNA under aggregation conditions, whereas we could not observe any SERS intensities under non-aggregation conditions.
Copyright ? 2012 Elsevier B.V. All rights reserved.
PMID:
22261178
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22261178
Aggregation effects of gold nanoparticles for single-base mismatch detection in influenza A (H1N1) DNA sequences using fluorescence and Raman measurements.
Ganbold EO, Kang T, Lee K, Lee SY, Joo SW.
Source
Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea.
Abstract
Aggregation effects of gold nanoparticles (AuNPs) were examined for the discrimination of single point mutations through the hybridization of oligonucleotides (25-50nM) modified with a fluorescent Texas red dye. The sequences of oligonucleotides were designed to detect the H1N1 virus gene. Single-base mismatch detection due to different adsorption propensities of oligonucleotides could be achieved using fluorescence quenching and surface-enhanced Raman scattering (SERS) properties of the dye. We observed that the addition of perfectly matched double stranded DNA (pmdsDNA), modified with the Texas red dye in the suspension of citrate-reduced AuNPs could increase fluorescence recovery intensities more substantially than either single-base mismatched double stranded DNA (sbmdsDNA) or single stranded DNA (ssDNA). We also tested DNA hybridization under both aggregation and near non-aggregation conditions for fluorescence measurements. A spectral difference in fluorescence intensity between pmdsDNA and sbmdsDNA appeared to be more discriminating under near non-aggregation than aggregation conditions. On the other hand, the SERS intensities of pmdsDNA and sbmdsDNA decreased more significantly than that of ssDNA under aggregation conditions, whereas we could not observe any SERS intensities under non-aggregation conditions.
Copyright ? 2012 Elsevier B.V. All rights reserved.
PMID:
22261178
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22261178