tetano
Editor, Senior Moderator
Adv Sci (Weinh)
. 2022 Jul 3;e2200956.
doi: 10.1002/advs.202200956. Online ahead of print.
Dynamic Alterations in the Respiratory Tract Microbiota of Patients with COVID-19 and its Association with Microbiota in the Gut
Yifei Shen[SUP] 1 2 3 [/SUP], Fei Yu[SUP] 1 2 3 [/SUP], Dan Zhang[SUP] 1 2 3 [/SUP], Qianda Zou[SUP] 1 [/SUP], Mengxiao Xie[SUP] 1 [/SUP], Xiao Chen[SUP] 1 2 3 [/SUP], Lingjun Yuan[SUP] 1 [/SUP], Bin Lou[SUP] 1 2 3 [/SUP], Guoliang Xie[SUP] 1 2 3 [/SUP], Ruonan Wang[SUP] 1 2 3 [/SUP], Xianzhi Yang[SUP] 1 2 3 [/SUP], Weizhen Chen[SUP] 1 2 3 [/SUP], Qi Wang[SUP] 1 2 [/SUP], Baihuan Feng[SUP] 1 2 3 [/SUP], Yun Teng[SUP] 1 2 3 [/SUP], Yuejiao Dong[SUP] 1 2 3 [/SUP], Li Huang[SUP] 1 2 3 [/SUP], Jiaqi Bao[SUP] 1 2 3 [/SUP], Dongsheng Han[SUP] 1 2 3 [/SUP], Chang Liu[SUP] 1 [/SUP], Wei Wu[SUP] 4 [/SUP], Xia Liu[SUP] 5 [/SUP], Longjiang Fan[SUP] 6 7 [/SUP], Michael P Timko[SUP] 8 [/SUP], Shufa Zheng[SUP] 1 2 3 4 [/SUP], Yu Chen[SUP] 1 2 3 4 [/SUP]
Affiliations
Abstract
The role of respiratory tract microbes and the relationship between respiratory tract and gut microbiomes in coronavirus disease 2019 (COVID-19) remain uncertain. Here, the metagenomes of sputum and fecal samples from 66 patients with COVID-19 at three stages of disease progression are sequenced. Respiratory tract, gut microbiome, and peripheral blood mononuclear cell (PBMC) samples are analyzed to compare the gut and respiratory tract microbiota of intensive care unit (ICU) and non-ICU (nICU) patients and determine relationships between respiratory tract microbiome and immune response. In the respiratory tract, significantly fewer Streptococcus, Actinomyces, Atopobium, and Bacteroides are found in ICU than in nICU patients, while Enterococcus and Candida increase. In the gut, significantly fewer Bacteroides are found in ICU patients, while Enterococcus increases. Significant positive correlations exist between relative microbiota abundances in the respiratory tract and gut. Defensin-related pathways in PBMCs are enhanced, and respiratory tract Streptococcus is reduced in patients with COVID-19. A respiratory tract-gut microbiota model identifies respiratory tract Streptococcus and Atopobium as the most prominent biomarkers distinguishing between ICU and nICU patients. The findings provide insight into the respiratory tract and gut microbial dynamics during COVID-19 progression, considering disease severity, potentially contributing to diagnosis, and treatment strategies.
Keywords: COVID-19; coronavirus disease 2019; disease severity; gut microbiota; intensive care unit; respiratory microbiota.
. 2022 Jul 3;e2200956.
doi: 10.1002/advs.202200956. Online ahead of print.
Dynamic Alterations in the Respiratory Tract Microbiota of Patients with COVID-19 and its Association with Microbiota in the Gut
Yifei Shen[SUP] 1 2 3 [/SUP], Fei Yu[SUP] 1 2 3 [/SUP], Dan Zhang[SUP] 1 2 3 [/SUP], Qianda Zou[SUP] 1 [/SUP], Mengxiao Xie[SUP] 1 [/SUP], Xiao Chen[SUP] 1 2 3 [/SUP], Lingjun Yuan[SUP] 1 [/SUP], Bin Lou[SUP] 1 2 3 [/SUP], Guoliang Xie[SUP] 1 2 3 [/SUP], Ruonan Wang[SUP] 1 2 3 [/SUP], Xianzhi Yang[SUP] 1 2 3 [/SUP], Weizhen Chen[SUP] 1 2 3 [/SUP], Qi Wang[SUP] 1 2 [/SUP], Baihuan Feng[SUP] 1 2 3 [/SUP], Yun Teng[SUP] 1 2 3 [/SUP], Yuejiao Dong[SUP] 1 2 3 [/SUP], Li Huang[SUP] 1 2 3 [/SUP], Jiaqi Bao[SUP] 1 2 3 [/SUP], Dongsheng Han[SUP] 1 2 3 [/SUP], Chang Liu[SUP] 1 [/SUP], Wei Wu[SUP] 4 [/SUP], Xia Liu[SUP] 5 [/SUP], Longjiang Fan[SUP] 6 7 [/SUP], Michael P Timko[SUP] 8 [/SUP], Shufa Zheng[SUP] 1 2 3 4 [/SUP], Yu Chen[SUP] 1 2 3 4 [/SUP]
Affiliations
- PMID: 35780499
- DOI: 10.1002/advs.202200956
Abstract
The role of respiratory tract microbes and the relationship between respiratory tract and gut microbiomes in coronavirus disease 2019 (COVID-19) remain uncertain. Here, the metagenomes of sputum and fecal samples from 66 patients with COVID-19 at three stages of disease progression are sequenced. Respiratory tract, gut microbiome, and peripheral blood mononuclear cell (PBMC) samples are analyzed to compare the gut and respiratory tract microbiota of intensive care unit (ICU) and non-ICU (nICU) patients and determine relationships between respiratory tract microbiome and immune response. In the respiratory tract, significantly fewer Streptococcus, Actinomyces, Atopobium, and Bacteroides are found in ICU than in nICU patients, while Enterococcus and Candida increase. In the gut, significantly fewer Bacteroides are found in ICU patients, while Enterococcus increases. Significant positive correlations exist between relative microbiota abundances in the respiratory tract and gut. Defensin-related pathways in PBMCs are enhanced, and respiratory tract Streptococcus is reduced in patients with COVID-19. A respiratory tract-gut microbiota model identifies respiratory tract Streptococcus and Atopobium as the most prominent biomarkers distinguishing between ICU and nICU patients. The findings provide insight into the respiratory tract and gut microbial dynamics during COVID-19 progression, considering disease severity, potentially contributing to diagnosis, and treatment strategies.
Keywords: COVID-19; coronavirus disease 2019; disease severity; gut microbiota; intensive care unit; respiratory microbiota.