tetano
Editor, Senior Moderator
Adv Mater Technol
. 2021 Sep 12;2100842.
doi: 10.1002/admt.202100842. Online ahead of print.
A Rapid Detection of COVID-19 Viral RNA in Human Saliva Using Electrical Double Layer-Gated Field-Effect Transistor-Based Biosensors
Akhil K Paulose[SUP] 1 [/SUP], Chih-Cheng Huang[SUP] 1 [/SUP], Po-Hsuan Chen[SUP] 1 [/SUP], Adarsh Tripathi[SUP] 2 [/SUP], Pin-Hsuan Chen[SUP] 3 [/SUP], Yu-Shan Huang[SUP] 1 [/SUP], Yu-Lin Wang[SUP] 1 3 [/SUP]
Affiliations
Abstract
In light of the swift outspread and considerable mortality, coronavirus disease 2019 (COVID-19) necessitates a rapid screening tool and a precise diagnosis. Saliva is considered as an alternative specimen to detect the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) since the viral load is comparable to what are found in a throat and a nasal cavity. The electrical double layer (EDL)-gated field-effect transistor-based biosensor (BioFET) emerges as a promising candidate for salivary COVID-19 tests due to a high sensitivity, a portable configuration, a label-free operation, and a matrix insensitivity. In this work, the authors utilize EDL-gated BioFETs to detect complementary DNAs (cDNAs) and viral RNAs with various testing conditions such as switches of probes, temperature treatments, and matrices. The selectivity is confirmed with cDNA and noncomplementary DNA (ncDNA), exhibiting an eightfold difference in electrical signals. The matrix insensitivity is evaluated, and BioFETs successfully validate the detection of SARS-CoV-2 N-gene RNA down to 1 fm in diluted human saliva with a 95°C- and a 25°C-treatment, respectively. This proposed system has a high potential to be deployed for an on-site COVID-19 screening, improving the disease control and benefitting frontline healthcare system.
Keywords: COVID‐19; EDL‐gated BioFET; SARS‐CoV‐2; portable biosensors; saliva‐based diagnostics; viral RNA screening.
. 2021 Sep 12;2100842.
doi: 10.1002/admt.202100842. Online ahead of print.
A Rapid Detection of COVID-19 Viral RNA in Human Saliva Using Electrical Double Layer-Gated Field-Effect Transistor-Based Biosensors
Akhil K Paulose[SUP] 1 [/SUP], Chih-Cheng Huang[SUP] 1 [/SUP], Po-Hsuan Chen[SUP] 1 [/SUP], Adarsh Tripathi[SUP] 2 [/SUP], Pin-Hsuan Chen[SUP] 3 [/SUP], Yu-Shan Huang[SUP] 1 [/SUP], Yu-Lin Wang[SUP] 1 3 [/SUP]
Affiliations
- PMID: 34901383
- PMCID: PMC8646907
- DOI: 10.1002/admt.202100842
Abstract
In light of the swift outspread and considerable mortality, coronavirus disease 2019 (COVID-19) necessitates a rapid screening tool and a precise diagnosis. Saliva is considered as an alternative specimen to detect the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) since the viral load is comparable to what are found in a throat and a nasal cavity. The electrical double layer (EDL)-gated field-effect transistor-based biosensor (BioFET) emerges as a promising candidate for salivary COVID-19 tests due to a high sensitivity, a portable configuration, a label-free operation, and a matrix insensitivity. In this work, the authors utilize EDL-gated BioFETs to detect complementary DNAs (cDNAs) and viral RNAs with various testing conditions such as switches of probes, temperature treatments, and matrices. The selectivity is confirmed with cDNA and noncomplementary DNA (ncDNA), exhibiting an eightfold difference in electrical signals. The matrix insensitivity is evaluated, and BioFETs successfully validate the detection of SARS-CoV-2 N-gene RNA down to 1 fm in diluted human saliva with a 95°C- and a 25°C-treatment, respectively. This proposed system has a high potential to be deployed for an on-site COVID-19 screening, improving the disease control and benefitting frontline healthcare system.
Keywords: COVID‐19; EDL‐gated BioFET; SARS‐CoV‐2; portable biosensors; saliva‐based diagnostics; viral RNA screening.