tetano
Editor, Senior Moderator
Int J Biol Macromol. 2012 Jan 13. [Epub ahead of print]
Adjuvanticity of compound polysaccharides on chickens against Newcastle disease and avian influenza vaccine.
Guo L, Wang D, Hu Y, Zhao X, Wang Y, Yang S, Wang J, Fan Y, Han G, Gao H.
Source
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Abstract
This study was conducted to evaluate the effects of compound polysaccharides (cPS) on the immune responses via chicken models. First, in screening experiment, a comprehensive analysis for immunomodulatory activity of four cPSs, including Astragalus polysaccharides (APS), Epimedium polysaccharides (EPS), sulfated APS (sAPS) and sulfated EPS (sEPS), was performed in vitro and in vivo. APS-sEPS was picked out having the best effect on lymphocyte proliferation and raising the antibody titers. Therefore, the adjuvanticities of APS-sEPS on Newcastle disease (ND) and avian influenza (AI) vaccine were further validated. Chickens were administrated with ND or AI vaccines containing APS-sEPS of 150, 100 and 50mg/kg, respectively, taking oil adjuvant vaccine as control. It was observed ND or AI antibody titers and lymphocyte proliferation were enhanced at 100mg/kg of APS-sEPS. In conclusion, appropriate dose of APS-sEPS may be a safe and efficacious immune stimulator candidate suitable for vaccines.
Copyright ? 2012. Published by Elsevier B.V.
PMID:
22266329
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22266329
Adjuvanticity of compound polysaccharides on chickens against Newcastle disease and avian influenza vaccine.
Guo L, Wang D, Hu Y, Zhao X, Wang Y, Yang S, Wang J, Fan Y, Han G, Gao H.
Source
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Abstract
This study was conducted to evaluate the effects of compound polysaccharides (cPS) on the immune responses via chicken models. First, in screening experiment, a comprehensive analysis for immunomodulatory activity of four cPSs, including Astragalus polysaccharides (APS), Epimedium polysaccharides (EPS), sulfated APS (sAPS) and sulfated EPS (sEPS), was performed in vitro and in vivo. APS-sEPS was picked out having the best effect on lymphocyte proliferation and raising the antibody titers. Therefore, the adjuvanticities of APS-sEPS on Newcastle disease (ND) and avian influenza (AI) vaccine were further validated. Chickens were administrated with ND or AI vaccines containing APS-sEPS of 150, 100 and 50mg/kg, respectively, taking oil adjuvant vaccine as control. It was observed ND or AI antibody titers and lymphocyte proliferation were enhanced at 100mg/kg of APS-sEPS. In conclusion, appropriate dose of APS-sEPS may be a safe and efficacious immune stimulator candidate suitable for vaccines.
Copyright ? 2012. Published by Elsevier B.V.
PMID:
22266329
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22266329