tetano
Editor, Senior Moderator
EMBO J. 2015 May 4. pii: e201590960. [Epub ahead of print]
[h=1]Adaptation of avian influenza A (H6N1) virus from avian to human receptor-binding preference.[/h] Wang F[SUP]1[/SUP], Qi J[SUP]2[/SUP], Bi Y[SUP]2[/SUP], Zhang W[SUP]2[/SUP], Wang M[SUP]1[/SUP], Zhang B[SUP]3[/SUP], Wang M[SUP]4[/SUP], Liu J[SUP]4[/SUP], Yan J[SUP]2[/SUP], Shi Y[SUP]5[/SUP], Gao GF[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The receptor-binding specificity of influenza A viruses is a major determinant for the host tropism of the virus, which enables interspecies transmission. In 2013, the first human case of infection with avian influenza A (H6N1) virus was reported in Taiwan. To gather evidence concerning the epidemic potential of H6 subtype viruses, we performed comprehensive analysis of receptor-binding properties of Taiwan-isolated H6 HAs from 1972 to 2013. We propose that the receptor-binding properties of Taiwan-isolated H6 HAs have undergone three major stages: initially avian receptor-binding preference, secondarily obtaining human receptor-binding capacity, and recently human receptor-binding preference, which has been confirmed by receptor-binding assessment of three representative virus isolates. Mutagenesis work revealed that E190V and G228S substitutions are important to acquire the human receptor-binding capacity, and the P186L substitution could reduce the binding to avian receptor. Further structural analysis revealed how the P186L substitution in the receptor-binding site of HA determines the receptor-binding preference change. We conclude that the human-infecting H6N1 evolved into a human receptor preference.
? 2015 The Authors.
[h=4]KEYWORDS:[/h] H6N1; crystal structure; hemagglutinin; receptor binding
PMID: 25940072 [PubMed - as supplied by publisher]
[h=1]Adaptation of avian influenza A (H6N1) virus from avian to human receptor-binding preference.[/h] Wang F[SUP]1[/SUP], Qi J[SUP]2[/SUP], Bi Y[SUP]2[/SUP], Zhang W[SUP]2[/SUP], Wang M[SUP]1[/SUP], Zhang B[SUP]3[/SUP], Wang M[SUP]4[/SUP], Liu J[SUP]4[/SUP], Yan J[SUP]2[/SUP], Shi Y[SUP]5[/SUP], Gao GF[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The receptor-binding specificity of influenza A viruses is a major determinant for the host tropism of the virus, which enables interspecies transmission. In 2013, the first human case of infection with avian influenza A (H6N1) virus was reported in Taiwan. To gather evidence concerning the epidemic potential of H6 subtype viruses, we performed comprehensive analysis of receptor-binding properties of Taiwan-isolated H6 HAs from 1972 to 2013. We propose that the receptor-binding properties of Taiwan-isolated H6 HAs have undergone three major stages: initially avian receptor-binding preference, secondarily obtaining human receptor-binding capacity, and recently human receptor-binding preference, which has been confirmed by receptor-binding assessment of three representative virus isolates. Mutagenesis work revealed that E190V and G228S substitutions are important to acquire the human receptor-binding capacity, and the P186L substitution could reduce the binding to avian receptor. Further structural analysis revealed how the P186L substitution in the receptor-binding site of HA determines the receptor-binding preference change. We conclude that the human-infecting H6N1 evolved into a human receptor preference.
? 2015 The Authors.
[h=4]KEYWORDS:[/h] H6N1; crystal structure; hemagglutinin; receptor binding
PMID: 25940072 [PubMed - as supplied by publisher]