tetano
Editor, Senior Moderator
Vet Microbiol. 2014 Jun 29. pii: S0378-1135(14)00310-1. doi: 10.1016/j.vetmic.2014.06.018. [Epub ahead of print]
Adaptation of a natural reassortant H5N2 avian influenza virus in mice.
Li Q1, Wang X1, Zhong L1, Wang X2, Sun Z1, Gao Z1, Cui Z1, Zhu J1, Gu M2, Liu X2, Liu X3.
Author information
Abstract
It is reported that the H5N2 highly pathogenic avian influenza virus A/chicken/Hebei/1102/2010 (HB10) is a natural reassortant between circulating H5N1 and endemic H9N2 influenza viruses. To evaluate the potential of its interspecies transmission, the wild-type HB10 was adapted in mice through serial lung passages. Increased virulence was detectable in 5 sequential lung passages in mice and a highly virulent mouse-adapted strain (HB10-MA) with a 50% mouse lethal dose of 102.5 50% egg infectious dose was obtained in 15 passages. The virulence and the replication efficiency of HB10-MA in mice were significantly higher than those of HB10 while HB10-MA grew faster and to significantly higher titers than HB10 in MDCK and A549 cells. Only five amino acid mutations in four viral proteins (HA-S227N, PB2-Q591K, PB2-D701N, PA-I554V and NP-R351K) of HB10-MA virus were found when compared with those of HB10, indicating that they may be responsible for the adaptation of the novel reassortant H5N2 avian influenza virus in mice with increased virulence and replication efficiency. The results in this study provide helpful insights into the pathogenic potential of novel reassortant H5N2 viruses to mammals that deserves further attentions.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Avian influenza virus; H5N2; H7N9; Mouse-adapted; Pathogenicity; Reassortant
PMID:
25037995
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25037995
Adaptation of a natural reassortant H5N2 avian influenza virus in mice.
Li Q1, Wang X1, Zhong L1, Wang X2, Sun Z1, Gao Z1, Cui Z1, Zhu J1, Gu M2, Liu X2, Liu X3.
Author information
Abstract
It is reported that the H5N2 highly pathogenic avian influenza virus A/chicken/Hebei/1102/2010 (HB10) is a natural reassortant between circulating H5N1 and endemic H9N2 influenza viruses. To evaluate the potential of its interspecies transmission, the wild-type HB10 was adapted in mice through serial lung passages. Increased virulence was detectable in 5 sequential lung passages in mice and a highly virulent mouse-adapted strain (HB10-MA) with a 50% mouse lethal dose of 102.5 50% egg infectious dose was obtained in 15 passages. The virulence and the replication efficiency of HB10-MA in mice were significantly higher than those of HB10 while HB10-MA grew faster and to significantly higher titers than HB10 in MDCK and A549 cells. Only five amino acid mutations in four viral proteins (HA-S227N, PB2-Q591K, PB2-D701N, PA-I554V and NP-R351K) of HB10-MA virus were found when compared with those of HB10, indicating that they may be responsible for the adaptation of the novel reassortant H5N2 avian influenza virus in mice with increased virulence and replication efficiency. The results in this study provide helpful insights into the pathogenic potential of novel reassortant H5N2 viruses to mammals that deserves further attentions.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Avian influenza virus; H5N2; H7N9; Mouse-adapted; Pathogenicity; Reassortant
PMID:
25037995
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25037995