tetano
Editor, Senior Moderator
Vaccine. 2016 Feb 19. pii: S0264-410X(16)00170-5. doi: 10.1016/j.vaccine.2016.02.028. [Epub ahead of print]
[h=1]Activation of cross-reactive mucosal T and B cell responses in human nasopharynx-associated lymphoid tissue in vitro by Modified Vaccinia Ankara-vectored influenza vaccines.[/h] Mullin J[SUP]1[/SUP], Ahmed MS[SUP]1[/SUP], Sharma R[SUP]2[/SUP], Upile N[SUP]2[/SUP], Beer H[SUP]2[/SUP], Achar P[SUP]3[/SUP], Puksuriwong S[SUP]1[/SUP], Ferrara F[SUP]4[/SUP], Temperton N[SUP]4[/SUP], McNamara P[SUP]5[/SUP], Lambe T[SUP]6[/SUP], Gilbert SC[SUP]6[/SUP], Zhang Q[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Recent efforts have been focused on the development of vaccines that could induce broad immunity against influenza virus, either through T cell responses to conserved internal antigens or B cell response to cross-reactive haemagglutinin (HA). We studied the capacity of Modified Vaccinia Ankara (MVA)-vectored influenza vaccines to induce cross-reactive immunity to influenza virus in human nasopharynx-associated lymphoid tissue (NALT) in vitro. Adenotonsillar cells were isolated and stimulated with MVA vaccines expressing either conserved nucleoprotein (NP) and matrix protein 1 (M1) (MVA-NP-M1) or pandemic H1N1 HA (MVA-pdmH1HA). The MVA vaccine uptake and expression, and T and B cell responses were analyzed. MVA-vectored vaccines were highly efficient infecting NALT and vaccine antigens were highly expressed by B cells. MVA-NP-M1 elicited T cell response with greater numbers of IFNγ-producing CD4+ T cells and tissue-resident memory T cells than controls. MVA-pdmH1HA induced cross-reactive anti-HA antibodies to a number of influenza subtypes, in an age-dependent manner. The cross-reactive antibodies include anti-avian H5N1 and mainly target HA2 domain.
[h=4]CONCLUSION:[/h] MVA vaccines are efficient in infecting NALT and the vaccine antigen is highly expressed by B cells. MVA vaccines expressing conserved influenza antigens induce cross-reactive T and B cell responses in human NALT in vitro, suggesting the potential as mucosal vaccines for broader immunity against influenza.
Copyright ? 2016. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Antibody response; Children and adults; Influenza vaccine; MVA-vectored vaccine; Mucosal immunity; Nasopharynx-associated lymphoid tissue (NALT)
PMID: 26902548 [PubMed - as supplied by publisher]
[h=1]Activation of cross-reactive mucosal T and B cell responses in human nasopharynx-associated lymphoid tissue in vitro by Modified Vaccinia Ankara-vectored influenza vaccines.[/h] Mullin J[SUP]1[/SUP], Ahmed MS[SUP]1[/SUP], Sharma R[SUP]2[/SUP], Upile N[SUP]2[/SUP], Beer H[SUP]2[/SUP], Achar P[SUP]3[/SUP], Puksuriwong S[SUP]1[/SUP], Ferrara F[SUP]4[/SUP], Temperton N[SUP]4[/SUP], McNamara P[SUP]5[/SUP], Lambe T[SUP]6[/SUP], Gilbert SC[SUP]6[/SUP], Zhang Q[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Recent efforts have been focused on the development of vaccines that could induce broad immunity against influenza virus, either through T cell responses to conserved internal antigens or B cell response to cross-reactive haemagglutinin (HA). We studied the capacity of Modified Vaccinia Ankara (MVA)-vectored influenza vaccines to induce cross-reactive immunity to influenza virus in human nasopharynx-associated lymphoid tissue (NALT) in vitro. Adenotonsillar cells were isolated and stimulated with MVA vaccines expressing either conserved nucleoprotein (NP) and matrix protein 1 (M1) (MVA-NP-M1) or pandemic H1N1 HA (MVA-pdmH1HA). The MVA vaccine uptake and expression, and T and B cell responses were analyzed. MVA-vectored vaccines were highly efficient infecting NALT and vaccine antigens were highly expressed by B cells. MVA-NP-M1 elicited T cell response with greater numbers of IFNγ-producing CD4+ T cells and tissue-resident memory T cells than controls. MVA-pdmH1HA induced cross-reactive anti-HA antibodies to a number of influenza subtypes, in an age-dependent manner. The cross-reactive antibodies include anti-avian H5N1 and mainly target HA2 domain.
[h=4]CONCLUSION:[/h] MVA vaccines are efficient in infecting NALT and the vaccine antigen is highly expressed by B cells. MVA vaccines expressing conserved influenza antigens induce cross-reactive T and B cell responses in human NALT in vitro, suggesting the potential as mucosal vaccines for broader immunity against influenza.
Copyright ? 2016. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Antibody response; Children and adults; Influenza vaccine; MVA-vectored vaccine; Mucosal immunity; Nasopharynx-associated lymphoid tissue (NALT)
PMID: 26902548 [PubMed - as supplied by publisher]