tetano
Editor, Senior Moderator
ACS Sens
. 2023 Apr 24.
doi: 10.1021/acssensors.3c00031. Online ahead of print.
Rapid and Accurate Detection of SARS-CoV-2 Using an iPad-Controlled, High-Throughput, Portable, and Multiplex Hi ve-Chip Platform ( Hi Cube)
Yuanshou Zhu[SUP] 1 2 [/SUP], Nelson Odiwuor[SUP] 3 4 [/SUP], Zigan Sha[SUP] 1 [/SUP], Yanjing Chen[SUP] 5 [/SUP], Ning Shao[SUP] 1 [/SUP], Xudong Wu[SUP] 6 [/SUP], Jianwei Chen[SUP] 1 [/SUP], Yang Li[SUP] 1 [/SUP], Shujuan Guo[SUP] 1 [/SUP], Dawei Shi[SUP] 7 [/SUP], Peng Liu[SUP] 6 [/SUP], Yan Zhang[SUP] 5 [/SUP], Hongping Wei[SUP] 3 4 [/SUP], Sheng-Ce Tao[SUP] 1 2 8 [/SUP]
Affiliations
Abstract
Rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the most effective measures to control the coronavirus disease 2019 (COVID-19) pandemic. However, there is still lack of an ideal detection platform capable of high sample throughput, portability, and multiplicity. Herein, by combining Hive-Chip (capillary microarray) and reverse transcriptional loop-mediated isothermal amplification (RT-LAMP), we developed an iPad-controlled, high-throughput (48 samples at one run), portable (smaller than a backpack), multiplex (monitoring 8 gene fragments in one reaction), and real-time detection platform for SARS-CoV-2 detection. This platform is composed of a portable Hive-Chip device (HiCube; 32.7 × 29.7 × 20 cm, 5 kg), custom-designed software, and optimized Hive-Chips. RT-LAMP primers targeting seven SARS-CoV-2 genes (S, E, M, N, ORF1ab, ORF3a, and ORF7a) and one positive control (human RNase P) were designed and prefixed in the Hive-Chip. On-chip RT-LAMP showed that the limit of detection (LOD) of SARS-CoV-2 synthetic RNAs is 1 copy/μL, and there is no cross-reaction among different target genes. The platform was validated by 100 clinical samples of SARS-CoV-2, and the results were highly consistent with those of the traditional real-time PCR assay. In addition, on-chip detection of 6 other respiratory pathogens showed no cross-reactivity. Overall, our platform has great potential for fast, accurate, and on-site detection of SARS-CoV-2.
Keywords: Hive-Chip platform; RT-LAMP; SARS-CoV-2; high-throughput and multiplex detection.
. 2023 Apr 24.
doi: 10.1021/acssensors.3c00031. Online ahead of print.
Rapid and Accurate Detection of SARS-CoV-2 Using an iPad-Controlled, High-Throughput, Portable, and Multiplex Hi ve-Chip Platform ( Hi Cube)
Yuanshou Zhu[SUP] 1 2 [/SUP], Nelson Odiwuor[SUP] 3 4 [/SUP], Zigan Sha[SUP] 1 [/SUP], Yanjing Chen[SUP] 5 [/SUP], Ning Shao[SUP] 1 [/SUP], Xudong Wu[SUP] 6 [/SUP], Jianwei Chen[SUP] 1 [/SUP], Yang Li[SUP] 1 [/SUP], Shujuan Guo[SUP] 1 [/SUP], Dawei Shi[SUP] 7 [/SUP], Peng Liu[SUP] 6 [/SUP], Yan Zhang[SUP] 5 [/SUP], Hongping Wei[SUP] 3 4 [/SUP], Sheng-Ce Tao[SUP] 1 2 8 [/SUP]
Affiliations
- PMID: 37093957
- DOI: 10.1021/acssensors.3c00031
Abstract
Rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the most effective measures to control the coronavirus disease 2019 (COVID-19) pandemic. However, there is still lack of an ideal detection platform capable of high sample throughput, portability, and multiplicity. Herein, by combining Hive-Chip (capillary microarray) and reverse transcriptional loop-mediated isothermal amplification (RT-LAMP), we developed an iPad-controlled, high-throughput (48 samples at one run), portable (smaller than a backpack), multiplex (monitoring 8 gene fragments in one reaction), and real-time detection platform for SARS-CoV-2 detection. This platform is composed of a portable Hive-Chip device (HiCube; 32.7 × 29.7 × 20 cm, 5 kg), custom-designed software, and optimized Hive-Chips. RT-LAMP primers targeting seven SARS-CoV-2 genes (S, E, M, N, ORF1ab, ORF3a, and ORF7a) and one positive control (human RNase P) were designed and prefixed in the Hive-Chip. On-chip RT-LAMP showed that the limit of detection (LOD) of SARS-CoV-2 synthetic RNAs is 1 copy/μL, and there is no cross-reaction among different target genes. The platform was validated by 100 clinical samples of SARS-CoV-2, and the results were highly consistent with those of the traditional real-time PCR assay. In addition, on-chip detection of 6 other respiratory pathogens showed no cross-reactivity. Overall, our platform has great potential for fast, accurate, and on-site detection of SARS-CoV-2.
Keywords: Hive-Chip platform; RT-LAMP; SARS-CoV-2; high-throughput and multiplex detection.