tetano
Editor, Senior Moderator
ACS Sens
. 2021 Jul 15.
doi: 10.1021/acssensors.1c00773. Online ahead of print.
Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis
Fanwu Gong[SUP] 1 [/SUP], Hua-Xing Wei[SUP] 2 [/SUP], Ji Qi[SUP] 3 [/SUP], Huan Ma[SUP] 1 [/SUP], Lianxin Liu[SUP] 4 [/SUP], Jianping Weng[SUP] 5 [/SUP], Xucai Zheng[SUP] 1 [/SUP], Qiangsheng Li[SUP] 1 [/SUP], Dan Zhao[SUP] 1 [/SUP], Haopeng Fang[SUP] 1 [/SUP], Liu Liu[SUP] 1 [/SUP], Hongliang He[SUP] 2 [/SUP], Cuichen Ma[SUP] 1 [/SUP], Jinglong Han[SUP] 6 [/SUP], Anyuan Sun[SUP] 2 [/SUP], Baolong Wang[SUP] 2 [/SUP], Tengchuan Jin[SUP] 1 [/SUP], Bowei Li[SUP] 3 [/SUP], Bofeng Li[SUP] 1 [/SUP]
Affiliations
Abstract
The spread of Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), resulting in a global pandemic with around four million deaths. Although there are a variety of nucleic acid-based tests for detecting SARS-CoV-2, these methods have a relatively high cost and require expensive supporting equipment. To overcome these limitations and improve the efficiency of SARS-CoV-2 diagnosis, we developed a microfluidic platform that collected serum by a pulling-force spinning top and paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) for quantitative IgA/IgM/IgG measurements in an instrument-free way. We further validated the paper-based microfluidic ELISA analysis of SARS-CoV-2 receptor-binding domain (RBD)-specific IgA/IgM/IgG antibodies from human blood samples as a good measurement with higher sensitivity compared with traditional IgM/IgG detection (99.7% vs 95.6%) for early illness onset patients. In conclusion, we provide an alternative solution for the diagnosis of SARS-CoV-2 in a portable manner by this smart integration of pulling-force spinning top and paper-based microfluidic immunoassay.
Keywords: COVID-19 diagnosis; SARS-Cov-2; anti-RBD antibody; microfluidic devices; paper-based ELISA; serum separation.
. 2021 Jul 15.
doi: 10.1021/acssensors.1c00773. Online ahead of print.
Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis
Fanwu Gong[SUP] 1 [/SUP], Hua-Xing Wei[SUP] 2 [/SUP], Ji Qi[SUP] 3 [/SUP], Huan Ma[SUP] 1 [/SUP], Lianxin Liu[SUP] 4 [/SUP], Jianping Weng[SUP] 5 [/SUP], Xucai Zheng[SUP] 1 [/SUP], Qiangsheng Li[SUP] 1 [/SUP], Dan Zhao[SUP] 1 [/SUP], Haopeng Fang[SUP] 1 [/SUP], Liu Liu[SUP] 1 [/SUP], Hongliang He[SUP] 2 [/SUP], Cuichen Ma[SUP] 1 [/SUP], Jinglong Han[SUP] 6 [/SUP], Anyuan Sun[SUP] 2 [/SUP], Baolong Wang[SUP] 2 [/SUP], Tengchuan Jin[SUP] 1 [/SUP], Bowei Li[SUP] 3 [/SUP], Bofeng Li[SUP] 1 [/SUP]
Affiliations
- PMID: 34263598
- DOI: 10.1021/acssensors.1c00773
Abstract
The spread of Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), resulting in a global pandemic with around four million deaths. Although there are a variety of nucleic acid-based tests for detecting SARS-CoV-2, these methods have a relatively high cost and require expensive supporting equipment. To overcome these limitations and improve the efficiency of SARS-CoV-2 diagnosis, we developed a microfluidic platform that collected serum by a pulling-force spinning top and paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) for quantitative IgA/IgM/IgG measurements in an instrument-free way. We further validated the paper-based microfluidic ELISA analysis of SARS-CoV-2 receptor-binding domain (RBD)-specific IgA/IgM/IgG antibodies from human blood samples as a good measurement with higher sensitivity compared with traditional IgM/IgG detection (99.7% vs 95.6%) for early illness onset patients. In conclusion, we provide an alternative solution for the diagnosis of SARS-CoV-2 in a portable manner by this smart integration of pulling-force spinning top and paper-based microfluidic immunoassay.
Keywords: COVID-19 diagnosis; SARS-Cov-2; anti-RBD antibody; microfluidic devices; paper-based ELISA; serum separation.