tetano
Editor, Senior Moderator
ACS Sens
. 2020 Jul 13.
doi: 10.1021/acssensors.0c00927. Online ahead of print.
Development of a sensitive immunochromatographic method using lanthanide fluorescent microsphere for rapid serodiagnosis of COVID-19
Meng Feng, Jun Chen, Jingna Xun, Ruixue Dai, Wang Zhao, Hongzhou Lu, Jin Xu, Li Chen, Guodong Sui, Xunjia Cheng
Abstract
The SARS-CoV-2 infection that caused the COVID-19 pandemic quickly spread worldwide within two months. Rapid diagnosis of the disease and isolation of patients is an effective way to prevent and control the spread of COVID-19. Therefore, a sensitive immunofluorescent assay method was developed for rapid detection of special IgM and IgG of COVID-19 in human serum within 10 mins. The recombinant nucleocapsid protein of 2019 novel coronavirus was used as capture antigen. Lanthanide, Eu(III) fluorescent microsphere was used to qualitative/semi-quantitative determine the solid phase immunochromatographic assay. A total of 28 clinical positive and 77 negative serum or plasma samples were included in the test. Based on the analysis of serum or plasma from COVID-19 patients and healthy peoples, the sensitivity and specificity of the immunochromatographic assay were calculated as 98.72% and 100% (IgG); 98.68% and 93.10% (IgM), respectively. The results demonstrated that rapid immunoassay has high sensitivity and specificity and was useful for rapid serodiagnosis of COVID-19.
. 2020 Jul 13.
doi: 10.1021/acssensors.0c00927. Online ahead of print.
Development of a sensitive immunochromatographic method using lanthanide fluorescent microsphere for rapid serodiagnosis of COVID-19
Meng Feng, Jun Chen, Jingna Xun, Ruixue Dai, Wang Zhao, Hongzhou Lu, Jin Xu, Li Chen, Guodong Sui, Xunjia Cheng
- PMID: 32660240
- DOI: 10.1021/acssensors.0c00927
Abstract
The SARS-CoV-2 infection that caused the COVID-19 pandemic quickly spread worldwide within two months. Rapid diagnosis of the disease and isolation of patients is an effective way to prevent and control the spread of COVID-19. Therefore, a sensitive immunofluorescent assay method was developed for rapid detection of special IgM and IgG of COVID-19 in human serum within 10 mins. The recombinant nucleocapsid protein of 2019 novel coronavirus was used as capture antigen. Lanthanide, Eu(III) fluorescent microsphere was used to qualitative/semi-quantitative determine the solid phase immunochromatographic assay. A total of 28 clinical positive and 77 negative serum or plasma samples were included in the test. Based on the analysis of serum or plasma from COVID-19 patients and healthy peoples, the sensitivity and specificity of the immunochromatographic assay were calculated as 98.72% and 100% (IgG); 98.68% and 93.10% (IgM), respectively. The results demonstrated that rapid immunoassay has high sensitivity and specificity and was useful for rapid serodiagnosis of COVID-19.