tetano
Editor, Senior Moderator
ACS Sens
. 2023 Apr 7.
doi: 10.1021/acssensors.2c02782. Online ahead of print.
A Multiplexed SERS Microassay for Accurate Detection of SARS-CoV-2 and Variants of Concern
Courtney Vedelago[SUP] 1 [/SUP], Junrong Li[SUP] 1 [/SUP], Kym Lowry[SUP] 2 3 [/SUP], Christopher Howard[SUP] 1 [/SUP], Alain Wuethrich[SUP] 1 [/SUP], Matt Trau[SUP] 1 4 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 variants play an important role in predicting patient outcome during postinfection, and with growing fears of COVID-19 reservoirs in domestic and wild animals, it is necessary to adapt detection systems for variant detection. However, variant-specific detection remains challenging. Surface-enhanced Raman scattering is a sensitive and multiplexing technique that allows the simultaneous detection of multiple targets for accurate identification. Here we propose the development of a multiplex SERS microassay to detect both the spike and nucleocapsid structural proteins of SARS-CoV-2. The designed SERS microassay integrates gold-silver hollow nanobox barcodes and electrohydrodynamically induced nanomixing which in combination enables highly specific and sensitive detection of SARS-CoV-2 and the S-protein epitopes to delineate between ancestral prevariant strains with the newer variants of concern, Delta and Omicron. The microassay allows detection from as low as 20 virus/μL and 50 pg/mL RBD protein and can clearly identify the virus among infected versus healthy nasopharyngeal swabs, with the potential to identify between variants. The detection of both S- and N-proteins of SARS-CoV-2 and the differentiation of variants on the SERS microassay can aid the early detection of COVID-19 to reduce transmission rates and lead into adequate treatments for those severely affected by the virus.
Keywords: microfluidic device; multiplex detection; nanomixing; severe acute respiratory syndrome coronavirus 2; surface-enhanced Raman spectroscopy.
. 2023 Apr 7.
doi: 10.1021/acssensors.2c02782. Online ahead of print.
A Multiplexed SERS Microassay for Accurate Detection of SARS-CoV-2 and Variants of Concern
Courtney Vedelago[SUP] 1 [/SUP], Junrong Li[SUP] 1 [/SUP], Kym Lowry[SUP] 2 3 [/SUP], Christopher Howard[SUP] 1 [/SUP], Alain Wuethrich[SUP] 1 [/SUP], Matt Trau[SUP] 1 4 [/SUP]
Affiliations
- PMID: 37026968
- DOI: 10.1021/acssensors.2c02782
Abstract
Severe acute respiratory syndrome coronavirus 2 variants play an important role in predicting patient outcome during postinfection, and with growing fears of COVID-19 reservoirs in domestic and wild animals, it is necessary to adapt detection systems for variant detection. However, variant-specific detection remains challenging. Surface-enhanced Raman scattering is a sensitive and multiplexing technique that allows the simultaneous detection of multiple targets for accurate identification. Here we propose the development of a multiplex SERS microassay to detect both the spike and nucleocapsid structural proteins of SARS-CoV-2. The designed SERS microassay integrates gold-silver hollow nanobox barcodes and electrohydrodynamically induced nanomixing which in combination enables highly specific and sensitive detection of SARS-CoV-2 and the S-protein epitopes to delineate between ancestral prevariant strains with the newer variants of concern, Delta and Omicron. The microassay allows detection from as low as 20 virus/μL and 50 pg/mL RBD protein and can clearly identify the virus among infected versus healthy nasopharyngeal swabs, with the potential to identify between variants. The detection of both S- and N-proteins of SARS-CoV-2 and the differentiation of variants on the SERS microassay can aid the early detection of COVID-19 to reduce transmission rates and lead into adequate treatments for those severely affected by the virus.
Keywords: microfluidic device; multiplex detection; nanomixing; severe acute respiratory syndrome coronavirus 2; surface-enhanced Raman spectroscopy.