tetano
Editor, Senior Moderator
ACS Nano
. 2022 Jan 18.
doi: 10.1021/acsnano.1c09371. Online ahead of print.
Noninvasive and Point-of-Care Surface-Enhanced Raman Scattering (SERS)-Based Breathalyzer for Mass Screening of Coronavirus Disease 2019 (COVID-19) under 5 min
Shi Xuan Leong[SUP] 1 [/SUP], Yong Xiang Leong[SUP] 1 [/SUP], Emily Xi Tan[SUP] 1 [/SUP], Howard Yi Fan Sim[SUP] 1 [/SUP], Charlynn Sher Lin Koh[SUP] 1 [/SUP], Yih Hong Lee[SUP] 1 [/SUP], Carice Chong[SUP] 1 [/SUP], Li Shiuan Ng[SUP] 1 [/SUP], Jaslyn Ru Ting Chen[SUP] 1 [/SUP], Desmond Wei Cheng Pang[SUP] 1 [/SUP], Lam Bang Thanh Nguyen[SUP] 1 [/SUP], Siew Kheng Boong[SUP] 1 [/SUP], Xuemei Han[SUP] 1 [/SUP], Ya-Chuan Kao[SUP] 1 [/SUP], Yi Heng Chua[SUP] 1 [/SUP], Gia Chuong Phan-Quang[SUP] 1 2 [/SUP], In Yee Phang[SUP] 2 [/SUP], Hiang Kwee Lee[SUP] 1 [/SUP], Mohammad Yazid Abdad[SUP] 3 4 5 [/SUP], Nguan Soon Tan[SUP] 6 7 [/SUP], Xing Yi Ling[SUP] 1 [/SUP]
Affiliations
Abstract
Population-wide surveillance of COVID-19 requires tests to be quick and accurate to minimize community transmissions. The detection of breath volatile organic compounds presents a promising option for COVID-19 surveillance but is currently limited by bulky instrumentation and inflexible analysis protocol. Here, we design a hand-held surface-enhanced Raman scattering-based breathalyzer to identify COVID-19 infected individuals in under 5 min, achieving >95% sensitivity and specificity across 501 participants regardless of their displayed symptoms. Our SERS-based breathalyzer harnesses key variations in vibrational fingerprints arising from interactions between breath metabolites and multiple molecular receptors to establish a robust partial least-squares discriminant analysis model for high throughput classifications. Crucially, spectral regions influencing classification show strong corroboration with reported potential COVID-19 breath biomarkers, both through experiment and in silico. Our strategy strives to spur the development of next-generation, noninvasive human breath diagnostic toolkits tailored for mass screening purposes.
Keywords: breath volatile organic compounds (BVOCs); breathomics; coronavirus disease 2019 (COVID-19); mass screening; surface-enhanced Raman scattering (SERS).
. 2022 Jan 18.
doi: 10.1021/acsnano.1c09371. Online ahead of print.
Noninvasive and Point-of-Care Surface-Enhanced Raman Scattering (SERS)-Based Breathalyzer for Mass Screening of Coronavirus Disease 2019 (COVID-19) under 5 min
Shi Xuan Leong[SUP] 1 [/SUP], Yong Xiang Leong[SUP] 1 [/SUP], Emily Xi Tan[SUP] 1 [/SUP], Howard Yi Fan Sim[SUP] 1 [/SUP], Charlynn Sher Lin Koh[SUP] 1 [/SUP], Yih Hong Lee[SUP] 1 [/SUP], Carice Chong[SUP] 1 [/SUP], Li Shiuan Ng[SUP] 1 [/SUP], Jaslyn Ru Ting Chen[SUP] 1 [/SUP], Desmond Wei Cheng Pang[SUP] 1 [/SUP], Lam Bang Thanh Nguyen[SUP] 1 [/SUP], Siew Kheng Boong[SUP] 1 [/SUP], Xuemei Han[SUP] 1 [/SUP], Ya-Chuan Kao[SUP] 1 [/SUP], Yi Heng Chua[SUP] 1 [/SUP], Gia Chuong Phan-Quang[SUP] 1 2 [/SUP], In Yee Phang[SUP] 2 [/SUP], Hiang Kwee Lee[SUP] 1 [/SUP], Mohammad Yazid Abdad[SUP] 3 4 5 [/SUP], Nguan Soon Tan[SUP] 6 7 [/SUP], Xing Yi Ling[SUP] 1 [/SUP]
Affiliations
- PMID: 35040314
- DOI: 10.1021/acsnano.1c09371
Abstract
Population-wide surveillance of COVID-19 requires tests to be quick and accurate to minimize community transmissions. The detection of breath volatile organic compounds presents a promising option for COVID-19 surveillance but is currently limited by bulky instrumentation and inflexible analysis protocol. Here, we design a hand-held surface-enhanced Raman scattering-based breathalyzer to identify COVID-19 infected individuals in under 5 min, achieving >95% sensitivity and specificity across 501 participants regardless of their displayed symptoms. Our SERS-based breathalyzer harnesses key variations in vibrational fingerprints arising from interactions between breath metabolites and multiple molecular receptors to establish a robust partial least-squares discriminant analysis model for high throughput classifications. Crucially, spectral regions influencing classification show strong corroboration with reported potential COVID-19 breath biomarkers, both through experiment and in silico. Our strategy strives to spur the development of next-generation, noninvasive human breath diagnostic toolkits tailored for mass screening purposes.
Keywords: breath volatile organic compounds (BVOCs); breathomics; coronavirus disease 2019 (COVID-19); mass screening; surface-enhanced Raman scattering (SERS).