tetano
Editor, Senior Moderator
ACS Infect Dis
. 2021 Mar 16.
doi: 10.1021/acsinfecdis.0c00725. Online ahead of print.
Multiplexed Serum Antibody Screening Platform Using Virus Extracts from Endemic Coronaviridae and SARS-CoV-2
Simon Fink[SUP] 1 [/SUP], Felix Ruoff[SUP] 1 [/SUP], Aaron Stahl[SUP] 1 [/SUP], Matthias Becker[SUP] 1 [/SUP], Philipp Kaiser[SUP] 1 [/SUP], Bjoern Traenkle[SUP] 2 [/SUP], Daniel Junker[SUP] 1 [/SUP], Frank Weise[SUP] 1 [/SUP], Natalia Ruetalo[SUP] 3 [/SUP], Sebastian H?rber[SUP] 4 5 6 [/SUP], Andreas Peter[SUP] 4 5 6 [/SUP], Annika Nelde[SUP] 7 8 9 [/SUP], Juliane Walz[SUP] 7 8 9 10 [/SUP], G?rard Krause[SUP] 11 12 [/SUP], Armin Baillot[SUP] 13 [/SUP], Katja Schenke-Layland[SUP] 1 9 14 15 [/SUP], Thomas O Joos[SUP] 1 [/SUP], Ulrich Rothbauer[SUP] 1 2 [/SUP], Nicole Schneiderhan-Marra[SUP] 1 [/SUP], Michael Schindler[SUP] 3 [/SUP], Markus F Templin[SUP] 1 [/SUP]
Affiliations
Abstract
The presence of antibodies against endemic coronaviruses has been linked to disease severity after SARS-CoV-2 infection. Assays capable of concomitantly detecting antibodies against endemic coronaviridae such as OC43, 229E, NL63, and SARS-CoV-2 may help to elucidate this question. We developed a serum screening platform using a bead-based Western blot system called DigiWest, capable of running hundreds of assays using microgram amounts of protein prepared directly from different viruses. Characterization of the immunoassay for detection of SARS-CoV-2 specific antibodies revealed a sensitivity of 90.3% and a diagnostic specificity of 98.1%. Concordance analysis with the SARS-CoV-2 immunoassays available by Roche, Siemens, and Euroimmun indicates comparable assay performances (Cohen's ? ranging from 0.8874 to 0.9508). Analogous assays for OC43, 229E, and NL63 were established and combined into one multiplex with the SARS-CoV-2 assay. Seroreactivity for different coronaviruses was detected with high incidence, and the multiplex assay was adapted for serum screening.
Keywords: COVID-19; Luminex; SARS-CoV-2; Western blot; endemic coronavirus; serology.
. 2021 Mar 16.
doi: 10.1021/acsinfecdis.0c00725. Online ahead of print.
Multiplexed Serum Antibody Screening Platform Using Virus Extracts from Endemic Coronaviridae and SARS-CoV-2
Simon Fink[SUP] 1 [/SUP], Felix Ruoff[SUP] 1 [/SUP], Aaron Stahl[SUP] 1 [/SUP], Matthias Becker[SUP] 1 [/SUP], Philipp Kaiser[SUP] 1 [/SUP], Bjoern Traenkle[SUP] 2 [/SUP], Daniel Junker[SUP] 1 [/SUP], Frank Weise[SUP] 1 [/SUP], Natalia Ruetalo[SUP] 3 [/SUP], Sebastian H?rber[SUP] 4 5 6 [/SUP], Andreas Peter[SUP] 4 5 6 [/SUP], Annika Nelde[SUP] 7 8 9 [/SUP], Juliane Walz[SUP] 7 8 9 10 [/SUP], G?rard Krause[SUP] 11 12 [/SUP], Armin Baillot[SUP] 13 [/SUP], Katja Schenke-Layland[SUP] 1 9 14 15 [/SUP], Thomas O Joos[SUP] 1 [/SUP], Ulrich Rothbauer[SUP] 1 2 [/SUP], Nicole Schneiderhan-Marra[SUP] 1 [/SUP], Michael Schindler[SUP] 3 [/SUP], Markus F Templin[SUP] 1 [/SUP]
Affiliations
- PMID: 33724771
- DOI: 10.1021/acsinfecdis.0c00725
Abstract
The presence of antibodies against endemic coronaviruses has been linked to disease severity after SARS-CoV-2 infection. Assays capable of concomitantly detecting antibodies against endemic coronaviridae such as OC43, 229E, NL63, and SARS-CoV-2 may help to elucidate this question. We developed a serum screening platform using a bead-based Western blot system called DigiWest, capable of running hundreds of assays using microgram amounts of protein prepared directly from different viruses. Characterization of the immunoassay for detection of SARS-CoV-2 specific antibodies revealed a sensitivity of 90.3% and a diagnostic specificity of 98.1%. Concordance analysis with the SARS-CoV-2 immunoassays available by Roche, Siemens, and Euroimmun indicates comparable assay performances (Cohen's ? ranging from 0.8874 to 0.9508). Analogous assays for OC43, 229E, and NL63 were established and combined into one multiplex with the SARS-CoV-2 assay. Seroreactivity for different coronaviruses was detected with high incidence, and the multiplex assay was adapted for serum screening.
Keywords: COVID-19; Luminex; SARS-CoV-2; Western blot; endemic coronavirus; serology.