tetano
Editor, Senior Moderator
ACS Appl Bio Mater
. 2021 Mar 15;4(3):2044-2051.
doi: 10.1021/acsabm.0c00968. Epub 2021 Jan 8.
Influenza A Viruses Enter Host Cells via Extracellular Ca [SUP]2+[/SUP] Influx-Involved Clathrin-Mediated Endocytosis
Meng-Ni Bao[SUP] 1 [/SUP], Li-Juan Zhang[SUP] 1 [/SUP], Bo Tang[SUP] 1 [/SUP], Dan-Dan Fu[SUP] 1 [/SUP], Jing Li[SUP] 1 [/SUP], Lei Du[SUP] 1 [/SUP], Yi-Ning Hou[SUP] 1 [/SUP], Zhi-Ling Zhang[SUP] 1 [/SUP], Hong-Wu Tang[SUP] 1 [/SUP], Dai-Wen Pang[SUP] 1 2 [/SUP]
Affiliations
Abstract
Influenza A virus (IAV) is internalized into its host cells by endocytosis, which involves many cellular proteins and molecules. In this study, we focus on the function of calcium ion (Ca[SUP]2+[/SUP]) in IAV endocytosis. We have found that IAV infection is accompanied by the increase in concentration of cytosolic Ca[SUP]2+[/SUP], which is mainly attributed to the influx of extracellular Ca[SUP]2+[/SUP]. When Ca[SUP]2+[/SUP] influx is abolished, IAV internalization will be markedly suppressed, but the virus attachment to its host cells will be unaffected. Extracellular Ca[SUP]2+[/SUP] influx is essential to the clathrin-mediated endocytosis (CME) of IAVs but dispensable to the clathrin-independent endocytosis of the virus and is dispensable to the CME of transferrin or low-density lipoprotein as a control. Ca[SUP]2+[/SUP] influx might participate in the dynamin-promoted membrane fission in the CME of IAVs. Our study highlights that IAVs enter host cells via extracellular Ca[SUP]2+[/SUP] influx-involved clathrin- and dynamin-dependent endocytosis, which will facilitate better understanding of IAV infection and development of anti-influenza drugs.
Keywords: Ca2+; clathrin; endocytosis; infection; influenza A virus.
. 2021 Mar 15;4(3):2044-2051.
doi: 10.1021/acsabm.0c00968. Epub 2021 Jan 8.
Influenza A Viruses Enter Host Cells via Extracellular Ca [SUP]2+[/SUP] Influx-Involved Clathrin-Mediated Endocytosis
Meng-Ni Bao[SUP] 1 [/SUP], Li-Juan Zhang[SUP] 1 [/SUP], Bo Tang[SUP] 1 [/SUP], Dan-Dan Fu[SUP] 1 [/SUP], Jing Li[SUP] 1 [/SUP], Lei Du[SUP] 1 [/SUP], Yi-Ning Hou[SUP] 1 [/SUP], Zhi-Ling Zhang[SUP] 1 [/SUP], Hong-Wu Tang[SUP] 1 [/SUP], Dai-Wen Pang[SUP] 1 2 [/SUP]
Affiliations
- PMID: 35014330
- DOI: 10.1021/acsabm.0c00968
Abstract
Influenza A virus (IAV) is internalized into its host cells by endocytosis, which involves many cellular proteins and molecules. In this study, we focus on the function of calcium ion (Ca[SUP]2+[/SUP]) in IAV endocytosis. We have found that IAV infection is accompanied by the increase in concentration of cytosolic Ca[SUP]2+[/SUP], which is mainly attributed to the influx of extracellular Ca[SUP]2+[/SUP]. When Ca[SUP]2+[/SUP] influx is abolished, IAV internalization will be markedly suppressed, but the virus attachment to its host cells will be unaffected. Extracellular Ca[SUP]2+[/SUP] influx is essential to the clathrin-mediated endocytosis (CME) of IAVs but dispensable to the clathrin-independent endocytosis of the virus and is dispensable to the CME of transferrin or low-density lipoprotein as a control. Ca[SUP]2+[/SUP] influx might participate in the dynamin-promoted membrane fission in the CME of IAVs. Our study highlights that IAVs enter host cells via extracellular Ca[SUP]2+[/SUP] influx-involved clathrin- and dynamin-dependent endocytosis, which will facilitate better understanding of IAV infection and development of anti-influenza drugs.
Keywords: Ca2+; clathrin; endocytosis; infection; influenza A virus.