tetano
Editor, Senior Moderator
J Clin Microbiol. 2016 Aug 24. pii: JCM.00610-16. [Epub ahead of print]
[h=1]Accurate diagnosis of avian respiratory viruses using multiplex PCR-based Luminex suspension microarray.[/h] Laamiri N[SUP]1[/SUP], F?llgren P[SUP]2[/SUP], Zohari S[SUP]2[/SUP], Ben Ali J[SUP]3[/SUP], Ghram A[SUP]4[/SUP], Leijon M[SUP]5[/SUP], Hmila I[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A novel oligonucleotide suspension microarray (Luminex? microsphere system) is developed for rapid diagnosis of avian respiratory viruses of major clinical importance. This test was optimized and validated with 70 clinical samples. The developed tool was accurate for high throughput detection and differentiation of the most important avian respiratory viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV) in single and mixed viral infections. A multiplex RT-PCR followed by a monoplex or a multiplex Luminex assays were realized using a Luminex-200 analyzer instrument. The sensitivity, the specificity and the reproducibility of the multiplex DNA suspension microarray system were evaluated. The results showed no significant differences in the median fluorescence intensity (MFI) value in monoplex and multiplex Luminex assays. The sensitivity and the specificity were proved to be completely concordant with monoplex real-time RT-PCR. We demonstrated that the multiplex DNA suspension microarray system is an accurate, a high-throughput and a relatively simple method for rapid diagnosis of the main respiratory viruses of poultry.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27558184 DOI: 10.1128/JCM.00610-16
[PubMed - as supplied by publisher]
[h=1]Accurate diagnosis of avian respiratory viruses using multiplex PCR-based Luminex suspension microarray.[/h] Laamiri N[SUP]1[/SUP], F?llgren P[SUP]2[/SUP], Zohari S[SUP]2[/SUP], Ben Ali J[SUP]3[/SUP], Ghram A[SUP]4[/SUP], Leijon M[SUP]5[/SUP], Hmila I[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A novel oligonucleotide suspension microarray (Luminex? microsphere system) is developed for rapid diagnosis of avian respiratory viruses of major clinical importance. This test was optimized and validated with 70 clinical samples. The developed tool was accurate for high throughput detection and differentiation of the most important avian respiratory viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV) in single and mixed viral infections. A multiplex RT-PCR followed by a monoplex or a multiplex Luminex assays were realized using a Luminex-200 analyzer instrument. The sensitivity, the specificity and the reproducibility of the multiplex DNA suspension microarray system were evaluated. The results showed no significant differences in the median fluorescence intensity (MFI) value in monoplex and multiplex Luminex assays. The sensitivity and the specificity were proved to be completely concordant with monoplex real-time RT-PCR. We demonstrated that the multiplex DNA suspension microarray system is an accurate, a high-throughput and a relatively simple method for rapid diagnosis of the main respiratory viruses of poultry.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27558184 DOI: 10.1128/JCM.00610-16
[PubMed - as supplied by publisher]