tetano
Editor, Senior Moderator
Access Microbiol
. 2024 Jul 22;6(7):000716.v3.
doi: 10.1099/acmi.0.000716.v3. eCollection 2024. In vitro comparison of viral replication and cytopathology induced by SARS-CoV-2 variants
Kruttika S Phadke[SUP] 1 2 [/SUP], Nathaniel B A Higdon[SUP] 1 2 [/SUP], Bryan H Bellaire[SUP] 1 2 [/SUP]
Affiliations
A myriad of coronaviruses cause diseases from a common cold to severe lung infections and pneumonia. SARS-CoV-2 was discovered to be the etiologic agent of the Coronavirus pandemic and many laboratory techniques were examined for virus culture and basic and applied research. Understanding the replication kinetics and characterizing the effect the virus has on different cell lines is crucial for developing in vitro studies. With the emergence of multiple variants of SARS-CoV-2, a comparison between their infectivity and replication in common cell lines will help give us a clear understanding of their characteristic differences in pathogenicity. In this study we compared the cytopathic effect and replication of Wild-Type (USA/WA1), Omicron (B.1.1.529), and Delta (B.1.617.2) variants on five different cell lines; VeroE6, VeroE6 cells expressing high endogenous ACE2, VeroE6 cells expressing human ACE2 and TMPRSS2, Calu3 cells highly expressing human ACE2 and A549 cells. This data will aid researchers with experimental planning and viral pathogenicity analysis and provide a baseline for testing any future variants.
Keywords: A549; BSL3; Calu3; SARS-CoV-2; cytopathic effect (CPE); vero E6; viral replication.
. 2024 Jul 22;6(7):000716.v3.
doi: 10.1099/acmi.0.000716.v3. eCollection 2024. In vitro comparison of viral replication and cytopathology induced by SARS-CoV-2 variants
Kruttika S Phadke[SUP] 1 2 [/SUP], Nathaniel B A Higdon[SUP] 1 2 [/SUP], Bryan H Bellaire[SUP] 1 2 [/SUP]
Affiliations
- PMID: 39130731
- PMCID: PMC11316578
- DOI: 10.1099/acmi.0.000716.v3
A myriad of coronaviruses cause diseases from a common cold to severe lung infections and pneumonia. SARS-CoV-2 was discovered to be the etiologic agent of the Coronavirus pandemic and many laboratory techniques were examined for virus culture and basic and applied research. Understanding the replication kinetics and characterizing the effect the virus has on different cell lines is crucial for developing in vitro studies. With the emergence of multiple variants of SARS-CoV-2, a comparison between their infectivity and replication in common cell lines will help give us a clear understanding of their characteristic differences in pathogenicity. In this study we compared the cytopathic effect and replication of Wild-Type (USA/WA1), Omicron (B.1.1.529), and Delta (B.1.617.2) variants on five different cell lines; VeroE6, VeroE6 cells expressing high endogenous ACE2, VeroE6 cells expressing human ACE2 and TMPRSS2, Calu3 cells highly expressing human ACE2 and A549 cells. This data will aid researchers with experimental planning and viral pathogenicity analysis and provide a baseline for testing any future variants.
Keywords: A549; BSL3; Calu3; SARS-CoV-2; cytopathic effect (CPE); vero E6; viral replication.