tetano
Editor, Senior Moderator
Immunology. 2015 Jun 10. doi: 10.1111/imm.12491. [Epub ahead of print]
[h=1]Abundance and specificity of influenza reactive circulating memory Tfh and non-Tfh CD4 T cells in healthy adults.[/h] Leddon SA[SUP]1[/SUP], Richards KA[SUP]1[/SUP], Treanor JJ[SUP]1[/SUP], Sant AJ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] CD4 T cell responses are functionally complex and regulate many aspects of innate and adaptive immunity. Follicular helper cells (Tfh) are CD4 T cells specialized to support B cell production of isotype-switched, high affinity antibody. Thus far, studies of Tfh cells in humans have focused on their differentiation requirements, with little research devoted to their antigen-specificity. Here, after separating circulating human memory CD4 T cells based on expression of CXCR5, a signature marker of Tfh, we have quantified and assayed the influenza protein antigen specificity of blood Tfh cells and CD4 T cells lacking this marker. Through the use of peptide pools derived from NP or HA and a panel of human donors, we have discovered that circulating Tfh cells preferentially recognize peptide epitopes from HA while cells lacking CXCR5 are enriched for specificity toward NP. These studies suggest that reactive CD4 T cells specific for distinct viral antigens may have generalized differences in their functional potential due to their previous stimulation history. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
PMID: 26094691 [PubMed - as supplied by publisher]
[h=1]Abundance and specificity of influenza reactive circulating memory Tfh and non-Tfh CD4 T cells in healthy adults.[/h] Leddon SA[SUP]1[/SUP], Richards KA[SUP]1[/SUP], Treanor JJ[SUP]1[/SUP], Sant AJ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] CD4 T cell responses are functionally complex and regulate many aspects of innate and adaptive immunity. Follicular helper cells (Tfh) are CD4 T cells specialized to support B cell production of isotype-switched, high affinity antibody. Thus far, studies of Tfh cells in humans have focused on their differentiation requirements, with little research devoted to their antigen-specificity. Here, after separating circulating human memory CD4 T cells based on expression of CXCR5, a signature marker of Tfh, we have quantified and assayed the influenza protein antigen specificity of blood Tfh cells and CD4 T cells lacking this marker. Through the use of peptide pools derived from NP or HA and a panel of human donors, we have discovered that circulating Tfh cells preferentially recognize peptide epitopes from HA while cells lacking CXCR5 are enriched for specificity toward NP. These studies suggest that reactive CD4 T cells specific for distinct viral antigens may have generalized differences in their functional potential due to their previous stimulation history. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
PMID: 26094691 [PubMed - as supplied by publisher]