tetano
Editor, Senior Moderator
Appl Environ Microbiol. 2014 Feb 28. [Epub ahead of print]
Abiotic factors affecting persistence of avian influenza virus in surface water from waterfowl habitats.
Keeler SP1, Dalton MS, Cressler AM, Berghaus RD, Stallknecht D.
Author information
Abstract
Avian influenza (AI) virus can remain infectious in water for months and virus contaminated surface water is considered to be a source of infection within wild waterfowl populations. Previous work has characterized the effect of pH, salinity, and temperature on viral persistence in water but a majority of that work has been done in modified distilled water. The objective of this study was to identify the abiotic factors that influence the duration of AI virus persistence in natural surface water. Surface water samples were collected from 38 waterfowl habitats distributed across the United States. Samples were submitted to the U.S. Geological Survey (USGS) National Water Quality Laboratory for chemical analysis and the University of Georgia (UGA) for viral reduction time analysis. Samples were filtered using 0.22μm filters and the duration of persistence within each water sample was determined for three wild-bird origin influenza A viruses at 10?C, 17?C, and 28?C. The effects of the surface-water physicochemical factors on the duration of AI viral persistence in laboratory experiments were evaluated using multivariable linear regression with robust standard errors. The duration of AI virus persistence in filtered water was determined to be longest in surface water with low temperature (<17?C), neutral-basic pH (7.0-8.5), low salinity (<0.5ppt), and low ammonia concentrations (<0.5mg/L). Our results also highlighted potential strain-related variation in the stability of AI virus in surface water. These results bring us closer to being able to predict the duration of AI virus viability in surface water of waterfowl habitats.
PMID:
24584247
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24584247
Abiotic factors affecting persistence of avian influenza virus in surface water from waterfowl habitats.
Keeler SP1, Dalton MS, Cressler AM, Berghaus RD, Stallknecht D.
Author information
Abstract
Avian influenza (AI) virus can remain infectious in water for months and virus contaminated surface water is considered to be a source of infection within wild waterfowl populations. Previous work has characterized the effect of pH, salinity, and temperature on viral persistence in water but a majority of that work has been done in modified distilled water. The objective of this study was to identify the abiotic factors that influence the duration of AI virus persistence in natural surface water. Surface water samples were collected from 38 waterfowl habitats distributed across the United States. Samples were submitted to the U.S. Geological Survey (USGS) National Water Quality Laboratory for chemical analysis and the University of Georgia (UGA) for viral reduction time analysis. Samples were filtered using 0.22μm filters and the duration of persistence within each water sample was determined for three wild-bird origin influenza A viruses at 10?C, 17?C, and 28?C. The effects of the surface-water physicochemical factors on the duration of AI viral persistence in laboratory experiments were evaluated using multivariable linear regression with robust standard errors. The duration of AI virus persistence in filtered water was determined to be longest in surface water with low temperature (<17?C), neutral-basic pH (7.0-8.5), low salinity (<0.5ppt), and low ammonia concentrations (<0.5mg/L). Our results also highlighted potential strain-related variation in the stability of AI virus in surface water. These results bring us closer to being able to predict the duration of AI virus viability in surface water of waterfowl habitats.
PMID:
24584247
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24584247