tetano
Editor, Senior Moderator
Virology. 2015 Mar 25;482:32-40. doi: 10.1016/j.virol.2015.03.004. [Epub ahead of print]
[h=1]A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells.[/h] Zhang H[SUP]1[/SUP], Han Q[SUP]1[/SUP], Ping X[SUP]2[/SUP], Li L[SUP]1[/SUP], Chang C[SUP]1[/SUP], Chen Z[SUP]3[/SUP], Shu Y[SUP]4[/SUP], Xu K[SUP]5[/SUP], Sun B[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Vaccination is the most effective way to prevent and control infection by influenza viruses, and a cell-culture-based vaccine production system is preferred as the future choice for the large-scale production of influenza vaccines. As one of the WHO-recommended cell lines for producing influenza vaccines, Vero cells do not efficiently support the growth of the current influenza A virus vaccine donor strain, the A/Puerto Rico/8/1934 (PR8) virus. In this study, a single mutation of K86R in the NS2 protein can sufficiently render the high-yielding property to the PR8 virus in Vero cells. Further analysis showed that the later steps in the virus replication cycle were accelerated by NS2[SUB]K86R[/SUB] mutation, which may relate to an enhanced interaction between NS2[SUB]K86R[/SUB] and the components of host factor F1Fo-ATPase, FoB and F1β. Because the NS2[SUB]K86R[/SUB] mutation does not increase PR8 virulence in either mice or embryonated eggs, the PR8-NS2[SUB]K86R[/SUB] virus could serve as a promising vaccine donor strain in Vero cells.
Copyright ? 2015 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza A virus; NS2; Vaccine donor virus; Vero cells
PMID: 25817403 [PubMed - as supplied by publisher]
[h=1]A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells.[/h] Zhang H[SUP]1[/SUP], Han Q[SUP]1[/SUP], Ping X[SUP]2[/SUP], Li L[SUP]1[/SUP], Chang C[SUP]1[/SUP], Chen Z[SUP]3[/SUP], Shu Y[SUP]4[/SUP], Xu K[SUP]5[/SUP], Sun B[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Vaccination is the most effective way to prevent and control infection by influenza viruses, and a cell-culture-based vaccine production system is preferred as the future choice for the large-scale production of influenza vaccines. As one of the WHO-recommended cell lines for producing influenza vaccines, Vero cells do not efficiently support the growth of the current influenza A virus vaccine donor strain, the A/Puerto Rico/8/1934 (PR8) virus. In this study, a single mutation of K86R in the NS2 protein can sufficiently render the high-yielding property to the PR8 virus in Vero cells. Further analysis showed that the later steps in the virus replication cycle were accelerated by NS2[SUB]K86R[/SUB] mutation, which may relate to an enhanced interaction between NS2[SUB]K86R[/SUB] and the components of host factor F1Fo-ATPase, FoB and F1β. Because the NS2[SUB]K86R[/SUB] mutation does not increase PR8 virulence in either mice or embryonated eggs, the PR8-NS2[SUB]K86R[/SUB] virus could serve as a promising vaccine donor strain in Vero cells.
Copyright ? 2015 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza A virus; NS2; Vaccine donor virus; Vero cells
PMID: 25817403 [PubMed - as supplied by publisher]