tetano
Editor, Senior Moderator
J Infect Dis. 2014 Sep 22. pii: jiu528. [Epub ahead of print]
A single immunization with an MVA-based influenza virus H7 vaccine affords protection in the H7N9 pneumonia ferret model.
Kreijtz JH1, Wiersma LC1, Gruyter De HL1, Vogelzang-van Trierum SE1, van Amerongen G2, Stittelaar KJ2, Fouchier RA3, Osterhaus AD1, Sutter G4, Rimmelzwaan GF1.
Author information
Abstract
Since the first reports in early 2013, over 440 human cases of infection with avian influenza A viruses of the H7N9 subtype have been reported including 122 fatalities. After the isolation of the first H7N9 viruses the nucleotide sequences became publically available. Based on the coding sequence of the influenza virus A/Shanghai/2/2013 HA gene, a codon-optimized gene was synthesized and cloned into a recombinant Modified Vaccinia virus Ankara (MVA). This MVA-H7-Sh2 viral vector was used to immunize ferrets and proved to be immunogenic, even after a single immunization. Subsequently, ferrets were challenged with influenza virus A/Anhui/1/2013 via the intratracheal route. Unprotected animals that were mock-vaccinated or that received empty vector, developed interstitial pneumonia characterized by a marked alveolitis, accompanied by loss of appetite, weight loss and heavy breathing. In contrast, MVA-H7-Sh2 immunized animals were protected from severe disease.
? The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
PMID:
25246535
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25246535
A single immunization with an MVA-based influenza virus H7 vaccine affords protection in the H7N9 pneumonia ferret model.
Kreijtz JH1, Wiersma LC1, Gruyter De HL1, Vogelzang-van Trierum SE1, van Amerongen G2, Stittelaar KJ2, Fouchier RA3, Osterhaus AD1, Sutter G4, Rimmelzwaan GF1.
Author information
Abstract
Since the first reports in early 2013, over 440 human cases of infection with avian influenza A viruses of the H7N9 subtype have been reported including 122 fatalities. After the isolation of the first H7N9 viruses the nucleotide sequences became publically available. Based on the coding sequence of the influenza virus A/Shanghai/2/2013 HA gene, a codon-optimized gene was synthesized and cloned into a recombinant Modified Vaccinia virus Ankara (MVA). This MVA-H7-Sh2 viral vector was used to immunize ferrets and proved to be immunogenic, even after a single immunization. Subsequently, ferrets were challenged with influenza virus A/Anhui/1/2013 via the intratracheal route. Unprotected animals that were mock-vaccinated or that received empty vector, developed interstitial pneumonia characterized by a marked alveolitis, accompanied by loss of appetite, weight loss and heavy breathing. In contrast, MVA-H7-Sh2 immunized animals were protected from severe disease.
? The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
PMID:
25246535
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25246535