Re: A/Shanghai/71T/2009 acquired E627K
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Shanghai isolated alpha-2 to complete the determination of influenza virus H1N1 </TD></TR><TR><TD height=20 align=middle></TD></TR></TBODY></TABLE></TD></TR><TR><TD colSpan=2 align=middle><SCRIPT>var status0_6562='';var curfontsize_6562=12;var curlineheight_6562=24;</SCRIPT><TABLE style="WORD-BREAK: break-all" border=0 cellSpacing=6 cellPadding=5 width="100%"><TBODY><TR><TD bgColor=#ffffff height=200 vAlign=top>
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记者10日从上海市公共卫生临床中心获悉，中心应急检测实验室已经成功分离出两株甲型H1N1流感病毒并完成全基因组序列测定。
10 reporters from the Shanghai Public Health Clinical Center was informed that the Center for emergency testing laboratories have successfully isolated two H1N1 Type A influenza virus and the completion of whole genome sequencing.
上海市公共卫生临床中心应急检测实验室承担国家网络实验室技术平台建设和传染病监测任务。
Shanghai Clinical Center for Public Health Emergency under the state testing laboratory network laboratory technology platform to monitor the task of building and infectious diseases.
2009年4月全球出现新型流感疫情后，应急检测实验室获卫生部批准可开展实验室检测和分离甲型H1N1流感病毒等工作。
The world in April 2009 after the emergence of new types of influenza epidemic, emergency testing laboratory approved by the Ministry of Health to carry out laboratory testing and H1N1 influenza viruses isolated alpha and so on.
自5月24日上海市首例输入性甲型H1N1流感疑似患者转送至市公卫临床中心进行医治后，中心应急检测实验室立即启动了核酸检测和病毒分离培养的工作。
Since May 24, Shanghai's first imported alpha in patients with suspected H1N1 influenza transferred to the city of clinical public health center for treatment after the emergency center immediately launched a testing laboratory of nucleic acid detection and virus isolation and culture of work.
考虑到甲型H1N1流感病毒生物学特性的不确定性，应急检测实验室同时采用细胞培养和鸡胚接种两种方法进行病毒分离。
Alpha, taking into account the biological characteristics of influenza virus H1N1 uncertainty, contingency testing laboratory using cell culture and chick embryo inoculation methods for virus isolation.
6月3日首先成功分离到5月23日自澳大利亚墨尔本回国患者标本的病毒，命名为A／Shanghai／37T／2009（H1N1）；6月7日另一株来自5月30日从美国回国患者的甲型H1N1流感病毒分离成功，病毒命名为A／Shanghai／71T／2009（H1N1）。
June 3 to the first successful separation of May 23 from Melbourne, Australia home specimens of patients with virus, named A/Shanghai/37T/2009 (H1N1); 6 other strains on day 7 from May 30 to return from the United States alpha in patients with H1N1 influenza virus isolation success of virus named A/Shanghai/71T/2009 (H1N1).
分离到病毒，上海市公卫临床中心研究人员立即开展了全长病毒基因的PCR扩增及测序工作，并完成病毒全基因组的PCR扩增和序列测定。
Separation of the virus, the Shanghai Public Health Clinical Center, researchers conducted a total length of the virus immediately for PCR amplification and gene sequencing, and the completion of the virus genome-wide PCR amplification and sequencing.
经过序列分析比较，两病毒分别与已公布的来自美国和澳大利亚的甲型H1N1流感病毒高度同源，表明为同一类病毒。
After sequence analysis, the two viruses were published from the United States and Australia, H1N1 Type A influenza viruses highly homologous to that for the same type of virus.
继北京、广东等地后，上海市公共卫生临床中心两株甲型H1N1流感病毒的分离为今后开展诊断试剂、疫苗、分子流行病学、药物开发和传播机制等各项相关研究工作提供了基础。
Following Beijing, Guangdong and other places, the Shanghai Public Health Clinical Center, H1N1 Type A influenza virus strains isolated for the future of diagnostic reagents, vaccines, molecular epidemiology, drug development and dissemination of the relevant mechanisms to provide a foundation for research work .
上述基因组序列信息已经提交全球公共序列数据库Genebank和全球流感共享数据库（GISAID），可以供全球的科学家用于甲型H1N1流感病毒的分析和监测。
The above-mentioned genome sequence information has been submitted to Genebank sequence database of global public and global influenza shared database (GISAID), available to scientists around the world for the H1N1 Type A influenza virus analysis and monitoring.
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