tetano
Editor, Senior Moderator
Bioanalysis. 2015 Feb;7(3):319-32. doi: 10.4155/bio.14.248.
[h=1]A rapid LC-MS/MS quantification of peramivir using a simple and inexpensive sample precipitation: application to PK.[/h] Wang ZZ[SUP]1[/SUP], Zhang M, Shang DW, Ni XJ, Hu JQ, Qiu C, Wen YG.
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]AIM:[/h] Peramivir is a newly approved selective neuraminidase inhibitor designed to inhibit influenza virus infection.
[h=4]METHODOLOGY/RESULTS:[/h] We report a robust and sensitive method utilizing simple precipitation extraction with LC-MS/MS for the high-throughput quantification. Addition of 0.06 M of ammonium formate and 0.1% formic acid in mobile phase could help reduce the matrix effect. This method uses 100 ?l of plasma and covers a linear concentration range from 5 to 10,000 ng/ml. Other validation parameters are also evaluated and meet regulatory expectations by US FDA guidelines.
[h=4]CONCLUSION:[/h] The developed HPLC-MS/MS method has been successfully applied to support a clinical pharmacokinetic study. The strategy presented here can be applied elsewhere and may be useful for other amphiphilic drugs.
PMID: 25697190 [PubMed - in process]
[h=1]A rapid LC-MS/MS quantification of peramivir using a simple and inexpensive sample precipitation: application to PK.[/h] Wang ZZ[SUP]1[/SUP], Zhang M, Shang DW, Ni XJ, Hu JQ, Qiu C, Wen YG.
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]AIM:[/h] Peramivir is a newly approved selective neuraminidase inhibitor designed to inhibit influenza virus infection.
[h=4]METHODOLOGY/RESULTS:[/h] We report a robust and sensitive method utilizing simple precipitation extraction with LC-MS/MS for the high-throughput quantification. Addition of 0.06 M of ammonium formate and 0.1% formic acid in mobile phase could help reduce the matrix effect. This method uses 100 ?l of plasma and covers a linear concentration range from 5 to 10,000 ng/ml. Other validation parameters are also evaluated and meet regulatory expectations by US FDA guidelines.
[h=4]CONCLUSION:[/h] The developed HPLC-MS/MS method has been successfully applied to support a clinical pharmacokinetic study. The strategy presented here can be applied elsewhere and may be useful for other amphiphilic drugs.
PMID: 25697190 [PubMed - in process]