tetano
Editor, Senior Moderator
J Med Virol. 2018 Feb 9. doi: 10.1002/jmv.25046. [Epub ahead of print]
[h=1]A point-of-care diagnostic system to influenza viruses using Chip-based Ultra-Fast PCR.[/h] Kwon SH[SUP]1[/SUP], Lee S[SUP]1[/SUP], Jang J[SUP]1[/SUP], Seo Y[SUP]2[/SUP], Lim HY[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In order to diagnose the infectious disease from clinical samples, the various protocols such as culturing microorganism, rapid diagnostic test using chromatographic method, ELISA, conventional PCR are developed. Since a novel strain of avian influenza can be cross-infected human as well as birds and livestock due to genetic reassortment, some strains of influenza such as H7N9 and H5N1 have emerged as a severe virus which can be threaten the health of poultry as well as human. Therefore we explored the development of simultaneously and rapid diagnostic tool for seasonal influenza (A/H1N1, A/H3N2, B) and highly pathogenic avian influenza (A/H5N1, A/H7N9). We analyzed the unique nucleotide sequences of influenza types including three seasonal influenza, A/H7N9, and A/H5N1, and distinguished each type of influenza and diagnosed through One Step RT-PCR. In the results, Chip-based PCR technique can be diagnosed rapidly and directly from naked eye with EvaGreen the influenza also respiratory specimens within 23 min 15sec, including reverse transcription. The Chip-based PCR is a point-of-care system, and it is expected to reduce diagnosis time and to develop a diagnostic kit. Furthermore the Chip based PCR technique can be used for high risk pathogen in bioterror and/or biological warfare in the field. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Chip-based PCR; EvaGreen; GeneChecker; Influenza subtyping; POCT
PMID: 29424457 DOI: 10.1002/jmv.25046
[h=1]A point-of-care diagnostic system to influenza viruses using Chip-based Ultra-Fast PCR.[/h] Kwon SH[SUP]1[/SUP], Lee S[SUP]1[/SUP], Jang J[SUP]1[/SUP], Seo Y[SUP]2[/SUP], Lim HY[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In order to diagnose the infectious disease from clinical samples, the various protocols such as culturing microorganism, rapid diagnostic test using chromatographic method, ELISA, conventional PCR are developed. Since a novel strain of avian influenza can be cross-infected human as well as birds and livestock due to genetic reassortment, some strains of influenza such as H7N9 and H5N1 have emerged as a severe virus which can be threaten the health of poultry as well as human. Therefore we explored the development of simultaneously and rapid diagnostic tool for seasonal influenza (A/H1N1, A/H3N2, B) and highly pathogenic avian influenza (A/H5N1, A/H7N9). We analyzed the unique nucleotide sequences of influenza types including three seasonal influenza, A/H7N9, and A/H5N1, and distinguished each type of influenza and diagnosed through One Step RT-PCR. In the results, Chip-based PCR technique can be diagnosed rapidly and directly from naked eye with EvaGreen the influenza also respiratory specimens within 23 min 15sec, including reverse transcription. The Chip-based PCR is a point-of-care system, and it is expected to reduce diagnosis time and to develop a diagnostic kit. Furthermore the Chip based PCR technique can be used for high risk pathogen in bioterror and/or biological warfare in the field. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Chip-based PCR; EvaGreen; GeneChecker; Influenza subtyping; POCT
PMID: 29424457 DOI: 10.1002/jmv.25046