tetano
Editor, Senior Moderator
Biosens Bioelectron. 2014 Sep 16;64C:311-317. doi: 10.1016/j.bios.2014.09.021. [Epub ahead of print]
A plasmon-assisted fluoro-immunoassay using gold nanoparticle-decorated carbon nanotubes for monitoring the influenza virus.
Lee J1, Ahmed SR2, Oh S3, Kim J3, Suzuki T4, Parmar K5, Park SS5, Lee J6, Park EY7.
Author information
Abstract
A plasmon-assisted fluoro-immunoassay (PAFI) was developed for the detection of the influenza virus by using Au nanoparticle (Au NP)-decorated carbon nanotubes (AuCNTs) that were synthesized using phytochemical composites at room temperature in deionized water. Specific antibodies (Abs) against the influenza virus were conjugated onto the surface of AuCNTs and cadmium telluride quantum dots (QDs), which had a photoluminescence intensity that varied as a function of virus concentration and a detection limit of 0.1pg/mL for all three types of influenza viruses examined. The clinically isolated influenza viruses (A/Yokohama/110/2009 (H3N2)) were detected in the range of 50-10,000PFU/mL, with a detection limit of 50PFU/mL. From a series of proof-of-concept and clinical experiments, the developed PAFI biosensing system provided robust signal production and enhancement, as well as an excellent selectivity and sensitivity for influenza viruses. This nanoparticle-based technique could be potentially developed as an efficient detection platform for the influenza virus.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
CdTe quantum dot; Gold nanoparticle-decorated carbon nanotube; Influenza virus detection platform; Plasmon-assisted fluoro-immunoassay; Plasmonic resonance energy transfer
PMID:
25240957
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25240957
A plasmon-assisted fluoro-immunoassay using gold nanoparticle-decorated carbon nanotubes for monitoring the influenza virus.
Lee J1, Ahmed SR2, Oh S3, Kim J3, Suzuki T4, Parmar K5, Park SS5, Lee J6, Park EY7.
Author information
Abstract
A plasmon-assisted fluoro-immunoassay (PAFI) was developed for the detection of the influenza virus by using Au nanoparticle (Au NP)-decorated carbon nanotubes (AuCNTs) that were synthesized using phytochemical composites at room temperature in deionized water. Specific antibodies (Abs) against the influenza virus were conjugated onto the surface of AuCNTs and cadmium telluride quantum dots (QDs), which had a photoluminescence intensity that varied as a function of virus concentration and a detection limit of 0.1pg/mL for all three types of influenza viruses examined. The clinically isolated influenza viruses (A/Yokohama/110/2009 (H3N2)) were detected in the range of 50-10,000PFU/mL, with a detection limit of 50PFU/mL. From a series of proof-of-concept and clinical experiments, the developed PAFI biosensing system provided robust signal production and enhancement, as well as an excellent selectivity and sensitivity for influenza viruses. This nanoparticle-based technique could be potentially developed as an efficient detection platform for the influenza virus.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
CdTe quantum dot; Gold nanoparticle-decorated carbon nanotube; Influenza virus detection platform; Plasmon-assisted fluoro-immunoassay; Plasmonic resonance energy transfer
PMID:
25240957
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25240957