tetano
Editor, Senior Moderator
Biomed Environ Sci. 2013 Jul;26(7):546-51. doi: 10.3967/0895-3988.2013.07.005.
A Novel Reassortant H3N8 Influenza Virus Isolated from Drinking Water for Duck in a Domestic Duck Farm in Poyang Lake Area.
Dong BB, Xu CL, Dong LB, Cheng HJ, Yang L, Zou SM, Chen M, Bai T, Zhang Y, Gao RB, Li XD, Shi JH, Yuan H, Yang J, Chen T, Zhu Y, Xiong Y, Yang S, Shu YL.
Source
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China; School of Public Health, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, China.
Abstract
OBJECTIVE:
To conduct a full genome sequence analysis for genetic characterization of an H3N8 influenza virus isolated from drinking water of a domestic duck farm in Poyang Lake area in 2011.
METHODS:
The virus was cultivated by specific pathogen free (SPF) chicken embryo eggs and was subtyped into hemagglutinin (HA) and neuraminidase (NA) by real-time PCR method. Eight gene segments were sequenced and phylogenetic analysis was conducted.
RESULTS:
The NA gene of this virus belongs to North American lineage; other seven genes belong to Eurasian lineage. Compared with the viruses containing NA gene, the PB2 and PB1 gene came from different clades. And this indicates that the virus was a novel reassortant genotype. The HA receptor binding preference was avian-like and the cleavage site sequence showed a low pathogenic feature. There was no drug resistance mutation of M2 protein. The mutations of Asn30Asp, and Thr215Ala of the M1 protein implied the potential of pathogenicity increase in mice.
CONCLUSION:
The finding of novel genotype of H3N8 virus in drinking water in this duck farm near Poyang Lake highlighted the importance of strengthening the surveillance of avian influenza in this region, which could contribute to pinpointing the influenza ecological relations among avian, swine, and human.
Copyright ? 2013 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.
KEYWORDS:
Avian influenza, H3N8, Sequence analysis
PMID:
23895699
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/23895699
A Novel Reassortant H3N8 Influenza Virus Isolated from Drinking Water for Duck in a Domestic Duck Farm in Poyang Lake Area.
Dong BB, Xu CL, Dong LB, Cheng HJ, Yang L, Zou SM, Chen M, Bai T, Zhang Y, Gao RB, Li XD, Shi JH, Yuan H, Yang J, Chen T, Zhu Y, Xiong Y, Yang S, Shu YL.
Source
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China; School of Public Health, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, China.
Abstract
OBJECTIVE:
To conduct a full genome sequence analysis for genetic characterization of an H3N8 influenza virus isolated from drinking water of a domestic duck farm in Poyang Lake area in 2011.
METHODS:
The virus was cultivated by specific pathogen free (SPF) chicken embryo eggs and was subtyped into hemagglutinin (HA) and neuraminidase (NA) by real-time PCR method. Eight gene segments were sequenced and phylogenetic analysis was conducted.
RESULTS:
The NA gene of this virus belongs to North American lineage; other seven genes belong to Eurasian lineage. Compared with the viruses containing NA gene, the PB2 and PB1 gene came from different clades. And this indicates that the virus was a novel reassortant genotype. The HA receptor binding preference was avian-like and the cleavage site sequence showed a low pathogenic feature. There was no drug resistance mutation of M2 protein. The mutations of Asn30Asp, and Thr215Ala of the M1 protein implied the potential of pathogenicity increase in mice.
CONCLUSION:
The finding of novel genotype of H3N8 virus in drinking water in this duck farm near Poyang Lake highlighted the importance of strengthening the surveillance of avian influenza in this region, which could contribute to pinpointing the influenza ecological relations among avian, swine, and human.
Copyright ? 2013 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.
KEYWORDS:
Avian influenza, H3N8, Sequence analysis
PMID:
23895699
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/23895699