tetano
Editor, Senior Moderator
nfluenza Other Respir Viruses. 2018 Sep 28. doi: 10.1111/irv.12613. [Epub ahead of print] [h=1]A newly developed tetraplex real time RT-PCR for simultaneous screening of influenza virus types A, B, C and D.[/h] Henritzi D[SUP]1[/SUP], Hoffmann B[SUP]1[/SUP], Wacheck S[SUP]2[/SUP], Pesch S[SUP]2[/SUP], Herrler G[SUP]3[/SUP], Beer M[SUP]1[/SUP], Harder TC[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Human- or avian-to-swine transmissions have founded several autonomously circulating influenza A virus (IAV) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (IBV) and C (ICV) in European swine, and of the recently detected novel animal influenza virus type D (IDV).
[h=4]OBJECTIVES:[/h] Development of a cost-effective diagnostic tool for large-scale surveillance programmes targeting all four influenza virus types.
[h=4]METHODS:[/h] An influenza ABCD tetraplex real-time RT-PCR (RT-qPCR) was developed in the frame of this study. A selection of reference virus strains, and more than 4,000 porcine samples from a passive IAV surveillance programme in European swine with acute respiratory disease were examined.
[h=4]RESULTS:[/h] Two IBV, a single IDV but no ICV infections were identified by tetraplex RT-qPCR. IBV and IDV results were confirmed by conventional RT-PCR and partial sequence analysis.
[h=4]CONCLUSIONS:[/h] The tetraplex RT-qPCR proved fit for purpose as a sensitive, specific and high throughput tool to study influenza virus transmission at the human-animal interface. Complementing close-meshed active virological and serological surveillance is required to better understand the true incidence and prevalence of influenza virus type B, C and D infections in swine. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] European surveillance; Influenza virus types A, B, C and D; multiplex RT-qPCR; swine
PMID: 30264926 DOI: 10.1111/irv.12613
Free full text
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Human- or avian-to-swine transmissions have founded several autonomously circulating influenza A virus (IAV) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (IBV) and C (ICV) in European swine, and of the recently detected novel animal influenza virus type D (IDV).
[h=4]OBJECTIVES:[/h] Development of a cost-effective diagnostic tool for large-scale surveillance programmes targeting all four influenza virus types.
[h=4]METHODS:[/h] An influenza ABCD tetraplex real-time RT-PCR (RT-qPCR) was developed in the frame of this study. A selection of reference virus strains, and more than 4,000 porcine samples from a passive IAV surveillance programme in European swine with acute respiratory disease were examined.
[h=4]RESULTS:[/h] Two IBV, a single IDV but no ICV infections were identified by tetraplex RT-qPCR. IBV and IDV results were confirmed by conventional RT-PCR and partial sequence analysis.
[h=4]CONCLUSIONS:[/h] The tetraplex RT-qPCR proved fit for purpose as a sensitive, specific and high throughput tool to study influenza virus transmission at the human-animal interface. Complementing close-meshed active virological and serological surveillance is required to better understand the true incidence and prevalence of influenza virus type B, C and D infections in swine. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] European surveillance; Influenza virus types A, B, C and D; multiplex RT-qPCR; swine
PMID: 30264926 DOI: 10.1111/irv.12613
Free full text