tetano
Editor, Senior Moderator
J Allergy Clin Immunol. 2018 May 28. pii: S0091-6749(18)30769-3. doi: 10.1016/j.jaci.2018.03.022. [Epub ahead of print]
[h=1]A Natural Killer T cell subset that protects against airway hyperreactivity.[/h] Chuang YT[SUP]1[/SUP], Leung K[SUP]2[/SUP], Chang YJ[SUP]3[/SUP], DeKruyff RH[SUP]4[/SUP], Savag PB[SUP]5[/SUP], Cruse R[SUP]6[/SUP], Benoit C[SUP]6[/SUP], Elewaut D[SUP]7[/SUP], Baumgarth N[SUP]8[/SUP], Umetsu DT[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Infection of suckling mice with influenza virus expands a CD4[SUP]-[/SUP]CD8[SUP]-[/SUP] double negative (DN) Natural Killer T (NKT) cell subpopulation that protects the mice as adults against allergen-induced airway hyperreactivity (AHR). However, this NKT cell subset has not been characterized and the underlying mechanisms of protection remain unknown.
[h=4]OBJECTIVE:[/h] We characterized this specific NKT cell subpopulation that developed during influenza infection in neonatal mice and that suppressed the subsequent development of AHR.
[h=4]METHODS:[/h] A cell surface marker was identified by comparing the mRNA expression profile of WT CD4[SUP]+[/SUP] NKT cells with that of suppressive Vα14 DN NKT cells. The marker-enriched NKT cell subset was then analyzed for its cytokine profile and its suppressive in vitro and in vivo abilities.
[h=4]RESULTS:[/h] We showed that DN NKT cells with high CD38 expression produced IFN-γ, but not IL-17, IL-4 or IL-13, and inhibited the development of AHR through contact-dependent suppression of helper CD4 T cell proliferation. The NKT subset expanded in the lungs of neonatal mice after infection with influenza and also after treatment of neonatal mice with Nu-α-GalCer, which effectively increased DN-CD38[SUP]hi[/SUP] NKT cell numbers.
[h=4]CONCLUSION:[/h] These results suggest that early/neonatal exposure to infection or antigenic challenge affects subsequent lung immunity by altering the cellular composition of cells in the lung, and that some subsets of NKT cells suppress AHR. These results provide a possible mechanism by which prior infections may protect against the development of allergic asthma and may be further explore as a protective measure for young children.
Copyright ? 2018. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] CD38; NKT subset; airway hyperreactivity; asthma; hygiene hypothesis; influenza
PMID: 29852257 DOI: 10.1016/j.jaci.2018.03.022
[h=1]A Natural Killer T cell subset that protects against airway hyperreactivity.[/h] Chuang YT[SUP]1[/SUP], Leung K[SUP]2[/SUP], Chang YJ[SUP]3[/SUP], DeKruyff RH[SUP]4[/SUP], Savag PB[SUP]5[/SUP], Cruse R[SUP]6[/SUP], Benoit C[SUP]6[/SUP], Elewaut D[SUP]7[/SUP], Baumgarth N[SUP]8[/SUP], Umetsu DT[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Infection of suckling mice with influenza virus expands a CD4[SUP]-[/SUP]CD8[SUP]-[/SUP] double negative (DN) Natural Killer T (NKT) cell subpopulation that protects the mice as adults against allergen-induced airway hyperreactivity (AHR). However, this NKT cell subset has not been characterized and the underlying mechanisms of protection remain unknown.
[h=4]OBJECTIVE:[/h] We characterized this specific NKT cell subpopulation that developed during influenza infection in neonatal mice and that suppressed the subsequent development of AHR.
[h=4]METHODS:[/h] A cell surface marker was identified by comparing the mRNA expression profile of WT CD4[SUP]+[/SUP] NKT cells with that of suppressive Vα14 DN NKT cells. The marker-enriched NKT cell subset was then analyzed for its cytokine profile and its suppressive in vitro and in vivo abilities.
[h=4]RESULTS:[/h] We showed that DN NKT cells with high CD38 expression produced IFN-γ, but not IL-17, IL-4 or IL-13, and inhibited the development of AHR through contact-dependent suppression of helper CD4 T cell proliferation. The NKT subset expanded in the lungs of neonatal mice after infection with influenza and also after treatment of neonatal mice with Nu-α-GalCer, which effectively increased DN-CD38[SUP]hi[/SUP] NKT cell numbers.
[h=4]CONCLUSION:[/h] These results suggest that early/neonatal exposure to infection or antigenic challenge affects subsequent lung immunity by altering the cellular composition of cells in the lung, and that some subsets of NKT cells suppress AHR. These results provide a possible mechanism by which prior infections may protect against the development of allergic asthma and may be further explore as a protective measure for young children.
Copyright ? 2018. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] CD38; NKT subset; airway hyperreactivity; asthma; hygiene hypothesis; influenza
PMID: 29852257 DOI: 10.1016/j.jaci.2018.03.022