tetano
Editor, Senior Moderator
J Vis Exp. 2018 Feb 23;(132). doi: 10.3791/56256.
[h=1]A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies.[/h] Bailey MJ[SUP]1[/SUP], Broecker F[SUP]2[/SUP], Leon PE[SUP]1[/SUP], Tan GS[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antibodies play a crucial role in coupling the innate and adaptive immune responses against viral pathogens through their antigen binding domains and Fc-regions. Here, we describe how to measure the activation of Fc effector functions by monoclonal antibodies targeting the influenza virus hemagglutinin with the use of a genetically engineered Jurkat cell line expressing an activating type 1 Fc-FcγR. Using this method, the contribution of specific Fc-FcγR interactions conferred by immunoglobulins can be determined using an in vitro assay.
PMID: 29553549 DOI: 10.3791/56256
[h=1]A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies.[/h] Bailey MJ[SUP]1[/SUP], Broecker F[SUP]2[/SUP], Leon PE[SUP]1[/SUP], Tan GS[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antibodies play a crucial role in coupling the innate and adaptive immune responses against viral pathogens through their antigen binding domains and Fc-regions. Here, we describe how to measure the activation of Fc effector functions by monoclonal antibodies targeting the influenza virus hemagglutinin with the use of a genetically engineered Jurkat cell line expressing an activating type 1 Fc-FcγR. Using this method, the contribution of specific Fc-FcγR interactions conferred by immunoglobulins can be determined using an in vitro assay.
PMID: 29553549 DOI: 10.3791/56256