A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses

[tetano]

Nat Microbiol. 2019 Apr 29. doi: 10.1038/s41564-019-0433-6. [Epub ahead of print]
[h=1]A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses.[/h] Takada K[SUP]1[/SUP], Kawakami C[SUP]2[/SUP], Fan S[SUP]3[/SUP], Chiba S[SUP]3[/SUP], Zhong G[SUP]3[/SUP], Gu C[SUP]3[/SUP], Shimizu K[SUP]2[/SUP], Takasaki S[SUP]1[/SUP], Sakai-Tagawa Y[SUP]1[/SUP], Lopes TJS[SUP]1,[/SUP][SUP]3[/SUP], Dutta J[SUP]4[/SUP], Khan Z[SUP]4[/SUP], Kriti D[SUP]4[/SUP], van Bakel H[SUP]4[/SUP], Yamada S[SUP]1[/SUP], Watanabe T[SUP]1[/SUP], Imai M[SUP]5[/SUP], Kawaoka Y[SUP]6,[/SUP][SUP]7,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]

[h=3]Abstract[/h] Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.


PMID: 31036910 DOI: 10.1038/s41564-019-0433-6