tetano
Editor, Senior Moderator
Antiviral Res. 2016 May 28. pii: S0166-3542(16)30129-2. doi: 10.1016/j.antiviral.2016.05.021. [Epub ahead of print]
[h=1]A critical role of T follicular helper cells in human mucosal anti-influenza response that can be enhanced by immunological adjuvant CpG-DNA.[/h] Aljurayyan AN[SUP]1[/SUP], Sharma R[SUP]2[/SUP], Upile N[SUP]2[/SUP], Beer H[SUP]2[/SUP], Vaughan C[SUP]2[/SUP], Xie C[SUP]2[/SUP], Achar P[SUP]3[/SUP], Ahmed MS[SUP]1[/SUP], McNamara P[SUP]4[/SUP], Gordon SB[SUP]5[/SUP], Zhang Q[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] T Follicular helper cells (T[SUB]FH[/SUB]) are considered critical for B cell antibody response, and recent efforts have focused on promoting T[SUB]FH[/SUB] in order to enhance vaccine efficacy. We studied the frequency and function of T[SUB]FH[/SUB] in nasopharynx-associated lymphoid tissues (NALT) from children and adults, and its role in anti-influenza antibody response following stimulation by a live-attenuated influenza vaccine (LAIV) or an inactivated seasonal virus antigen (sH1N1). We further studied whether CpG-DNA promotes T[SUB]FH[/SUB] and by which enhances anti-influenza response. We showed NALT from children aged 1.5-10 years contained abundant T[SUB]FH[/SUB], suggesting efficient priming of T[SUB]FH[/SUB] during early childhood. Stimulation by LAIV induced a marked increase in T[SUB]FH[/SUB] that correlated with a strong production of anti-hemagglutinin (HA) IgA/IgG/IgM antibodies in tonsillar cells. Stimulation by the inactivated sH1N1 antigen induced a small increase in T[SUB]FH[/SUB] which was markedly enhanced by CpG-DNA, accompanied by enhanced anti-HA antibody responses. In B cell co-culture experiment, anti-HA responses were only seen in the presence of T[SUB]FH,[/SUB] and addition of plasmacytoid dendritic cell to T[SUB]FH[/SUB]-B cell co-culture enhanced the T[SUB]FH[/SUB]-mediated antibody production following CpG-DNA and sH1N1 antigen stimulation. Induction of T[SUB]FH[/SUB] differentiation from na?ve T cells was also shown following the stimulation. Our results support a critical role of T[SUB]FH[/SUB] in human mucosal anti-influenza antibody response. Use of an adjuvant such as CpG-DNA that has the capacity to promote T[SUB]FH[/SUB] by which to enhance antigen-induced antibody responses in NALT tissue may have important implications for future vaccination strategies against respiratory pathogens.
Copyright ? 2016. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Anti-hemagglutinin (HA) antibody response; Children and adults; CpG-DNA; Influenza vaccine; Influenza virus; Nasopharynx-associated lymphoid tissues (NALT); T follicular helper cell (T(FH))
PMID: 27247060 [PubMed - as supplied by publisher]
[h=1]A critical role of T follicular helper cells in human mucosal anti-influenza response that can be enhanced by immunological adjuvant CpG-DNA.[/h] Aljurayyan AN[SUP]1[/SUP], Sharma R[SUP]2[/SUP], Upile N[SUP]2[/SUP], Beer H[SUP]2[/SUP], Vaughan C[SUP]2[/SUP], Xie C[SUP]2[/SUP], Achar P[SUP]3[/SUP], Ahmed MS[SUP]1[/SUP], McNamara P[SUP]4[/SUP], Gordon SB[SUP]5[/SUP], Zhang Q[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] T Follicular helper cells (T[SUB]FH[/SUB]) are considered critical for B cell antibody response, and recent efforts have focused on promoting T[SUB]FH[/SUB] in order to enhance vaccine efficacy. We studied the frequency and function of T[SUB]FH[/SUB] in nasopharynx-associated lymphoid tissues (NALT) from children and adults, and its role in anti-influenza antibody response following stimulation by a live-attenuated influenza vaccine (LAIV) or an inactivated seasonal virus antigen (sH1N1). We further studied whether CpG-DNA promotes T[SUB]FH[/SUB] and by which enhances anti-influenza response. We showed NALT from children aged 1.5-10 years contained abundant T[SUB]FH[/SUB], suggesting efficient priming of T[SUB]FH[/SUB] during early childhood. Stimulation by LAIV induced a marked increase in T[SUB]FH[/SUB] that correlated with a strong production of anti-hemagglutinin (HA) IgA/IgG/IgM antibodies in tonsillar cells. Stimulation by the inactivated sH1N1 antigen induced a small increase in T[SUB]FH[/SUB] which was markedly enhanced by CpG-DNA, accompanied by enhanced anti-HA antibody responses. In B cell co-culture experiment, anti-HA responses were only seen in the presence of T[SUB]FH,[/SUB] and addition of plasmacytoid dendritic cell to T[SUB]FH[/SUB]-B cell co-culture enhanced the T[SUB]FH[/SUB]-mediated antibody production following CpG-DNA and sH1N1 antigen stimulation. Induction of T[SUB]FH[/SUB] differentiation from na?ve T cells was also shown following the stimulation. Our results support a critical role of T[SUB]FH[/SUB] in human mucosal anti-influenza antibody response. Use of an adjuvant such as CpG-DNA that has the capacity to promote T[SUB]FH[/SUB] by which to enhance antigen-induced antibody responses in NALT tissue may have important implications for future vaccination strategies against respiratory pathogens.
Copyright ? 2016. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Anti-hemagglutinin (HA) antibody response; Children and adults; CpG-DNA; Influenza vaccine; Influenza virus; Nasopharynx-associated lymphoid tissues (NALT); T follicular helper cell (T(FH))
PMID: 27247060 [PubMed - as supplied by publisher]